AC   MF_03118;
DC   Protein; auto
TR   HAMAP; MF_03118; -; 1; level=0
XX
Names: Salvage_MtnBC
XX
ID   MTBC
DE   RecName: Full=Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1;
DE   Includes:
DE     RecName: Full=Methylthioribulose-1-phosphate dehydratase;
DE              Short=MTRu-1-P dehydratase;
DE              EC=4.2.1.109;
DE   Includes:
DE     RecName: Full=Enolase-phosphatase E1;
DE              EC=3.1.3.77;
DE     AltName: Full=2,3-diketo-5-methylthio-1-phosphopentane phosphatase;
XX
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-methyl-5-thio-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-
CC         dioxopentyl phosphate + H2O; Xref=Rhea:RHEA:15549, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:58548, ChEBI:CHEBI:58828; EC=4.2.1.109;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-
CC         dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate;
CC         Xref=Rhea:RHEA:21700, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:49252, ChEBI:CHEBI:58828; EC=3.1.3.77;
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC       Note=Binds 1 zinc ion per subunit.;
case <FTGroup:1>
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC       Note=Binds 1 Mg(2+) ion per subunit.;
end case
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage
CC       pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate:
CC       step 2/6.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage
CC       pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate:
CC       step 3/6.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via salvage
CC       pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate:
CC       step 4/6.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the aldolase class II
CC       family. MtnB subfamily.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the HAD-like
CC       hydrolase superfamily. MasA/MtnC family.
XX
DR   Pfam; PF00596; Aldolase_II; 1; trigger=no
DR   Pfam; PF00702; Hydrolase; 1; trigger=no
DR   NCBIfam; TIGR01691; enolase-ppase; 1; trigger=no
DR   NCBIfam; TIGR01549; HAD-SF-IA-v1; 1; trigger=no
DR   NCBIfam; TIGR03328; salvage_mtnB; 1; trigger=no
XX
KW   Amino-acid biosynthesis
KW   Hydrolase
KW   Lyase
case <FTGroup:1>
KW   Magnesium
end case
KW   Metal-binding
KW   Methionine biosynthesis
KW   Multifunctional enzyme
KW   Zinc
XX
case <FTGroup:1>
GO   GO:0000287; F:magnesium ion binding
end case
GO   GO:0008270; F:zinc ion binding
GO   GO:0043715; F:2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity
GO   GO:0043716; F:2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity
GO   GO:0046570; F:methylthioribulose 1-phosphate dehydratase activity
GO   GO:0019284; P:L-methionine salvage from S-adenosylmethionine
XX
FT   From: MTBC_ARATH (Q9FN41)
FT   REGION          Nter..237
FT                   /note="Methylthioribulose-1-phosphate dehydratase"
FT   REGION          268..Cter
FT                   /note="Enolase-phosphatase E1"
FT   BINDING         406..407
FT                   /ligand="substrate"
FT                   /ligand_label="2"
FT                   /ligand_note="for enolase-phosphatase activity"
FT   Condition: S-S
FT   ACT_SITE        152
FT                   /note="Proton donor/acceptor; for methylthioribulose-1-
FT                   phosphate dehydratase activity"
FT   Condition: E
FT   BINDING         127
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Condition: H
FT   BINDING         129
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Condition: H
FT   BINDING         202
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Condition: H
FT   BINDING         109
FT                   /ligand="substrate"
FT                   /ligand_label="1"
FT                   /ligand_note="for methylthioribulose-1-phosphate
FT                   dehydratase activity"
FT   Condition: C
FT   BINDING         271
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: D
FT   BINDING         273
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: E
FT   BINDING         466
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT   Group: 1; Condition: D
FT   BINDING         440
FT                   /ligand="substrate"
FT                   /ligand_label="2"
FT                   /ligand_note="for enolase-phosphatase activity"
FT   Condition: K
XX
Size: 504-531;
Related: MF_03116; MF_03117;
Template: Q9FN41;
Scope:
 Eukaryota; Viridiplantae
Fusion:
 Nter: None
 Cter: None
Duplicate: in VITVI
Plasmid: None
Comments: None
XX
# Revision 1.15 2023/06/01
//