AC MF_03130; DC Protein; auto TR HAMAP; MF_03130; -; 1; level=0 XX Names: mec17 XX ID ATAT case DE RecName: Full=Alpha-tubulin N-acetyltransferase 1; DE Short=Alpha-TAT; DE Short=Alpha-TAT1; DE Short=TAT; DE EC=2.3.1.108; DE AltName: Full=Acetyltransferase mec-17 homolog; else case DE RecName: Full=Alpha-tubulin N-acetyltransferase; DE Short=Alpha-TAT; DE Short=TAT; DE EC=2.3.1.108; else DE RecName: Full=Alpha-tubulin N-acetyltransferase; DE Short=Alpha-TAT; DE Short=TAT; DE EC=2.3.1.108; DE AltName: Full=Acetyltransferase mec-17 homolog; end case case GN Name=mec-17; else case GN Name=ATAT1; Synonyms=MEC17; end case XX case CC -!- FUNCTION: Specifically acetylates 'Lys-40' in alpha-tubulin on the CC lumenal side of microtubules. Promotes microtubule destabilization and CC accelerates microtubule dynamics; this activity may be independent of CC acetylation activity. Acetylates alpha-tubulin with a slow enzymatic CC rate, due to a catalytic site that is not optimized for acetyl CC transfer. Enters the microtubule through each end and diffuses quickly CC throughout the lumen of microtubules. Acetylates only long/old CC microtubules because of its slow acetylation rate since it does not CC have time to act on dynamically unstable microtubules before the enzyme CC is released. Required for normal sperm flagellar function. Promotes CC directional cell locomotion and chemotaxis, through AP2A2-dependent CC acetylation of alpha-tubulin at clathrin-coated pits that are CC concentrated at the leading edge of migrating cells. May facilitate CC primary cilium assembly. else case CC -!- FUNCTION: Specifically acetylates 'Lys-40' in alpha-tubulin on the CC lumenal side of microtubules. Promotes microtubule destabilization and CC accelerates microtubule dynamics; this activity may be independent of CC acetylation activity. Acetylates alpha-tubulin with a slow enzymatic CC rate, due to a catalytic site that is not optimized for acetyl CC transfer. Enters the microtubule through each end and diffuses quickly CC throughout the lumen of microtubules. Acetylates only long/old CC microtubules because of its slow acetylation rate since it does not CC have time to act on dynamically unstable microtubules before the enzyme CC is released. May be involved in neuron development. else case CC -!- FUNCTION: Specifically acetylates 'Lys-40' in alpha-tubulin/mec-12 on CC the lumenal side of microtubules. Promotes microtubule destabilization CC and accelerates microtubule dynamics; this activity may be independent CC of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic CC rate, due to a catalytic site that is not optimized for acetyl CC transfer. Enters the microtubule through each end and diffuses quickly CC throughout the lumen of microtubules. Acetylates only long/old CC microtubules because of its slow acetylation rate since it does not CC have time to act on dynamically unstable microtubules before the enzyme CC is released. Required for the maintenance of touch receptor neurons and CC possibly other type of neurons involved in locomotion. else CC -!- FUNCTION: Specifically acetylates 'Lys-40' in alpha-tubulin on the CC lumenal side of microtubules. Promotes microtubule destabilization and CC accelerates microtubule dynamics; this activity may be independent of CC acetylation activity. Acetylates alpha-tubulin with a slow enzymatic CC rate, due to a catalytic site that is not optimized for acetyl CC transfer. Enters the microtubule through each end and diffuses quickly CC throughout the lumen of microtubules. Acetylates only long/old CC microtubules because of its slow acetylation rate since it does not CC have time to act on dynamically unstable microtubules before the enzyme CC is released. end case CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + L-lysyl-[alpha-tubulin] = CoA + H(+) + N(6)- CC acetyl-L-lysyl-[alpha-tubulin]; Xref=Rhea:RHEA:15277, Rhea:RHEA- CC COMP:11278, Rhea:RHEA-COMP:11279, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:29969, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, CC ChEBI:CHEBI:61930; EC=2.3.1.108; case CC -!- SUBUNIT: Component of the BBSome complex. Interacts with AP2 alpha- CC adaptins, including AP2A2, but not with AP1 gamma-adaptin CC (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin CC acetylation, hence clathrin-coated pits are sites of microtubule CC acetylation. CC -!- PTM: Autoacetylation strongly increases tubulin acetylation. end case case CC -!- SUBCELLULAR LOCATION: Cytoplasm. Membrane, clathrin-coated pit. Cell CC junction, focal adhesion. Cell projection, axon. Cytoplasm, CC cytoskeleton. Cytoplasm, cytoskeleton, spindle. end case CC -!- SIMILARITY: Belongs to the acetyltransferase ATAT1 family. XX DR Pfam; PF05301; Mec-17; 1; trigger=no DR PROSITE; PS51730; GNAT_ATAT; 1; trigger=yes XX case KW Acetylation end case case KW Cell junction KW Cell projection KW Coated pit KW Cytoplasm KW Cytoskeleton KW Membrane end case KW Acyltransferase KW Transferase XX GO GO:0019799; F:tubulin N-acetyltransferase activity GO GO:0071929; P:alpha-tubulin acetylation GO GO:0070507; P:regulation of microtubule cytoskeleton organization case and not and not GO GO:0048666; P:neuron development end case XX FT From: ATAT_HUMAN (Q5SQI0) FT BINDING 124..137 FT /ligand="acetyl-CoA" FT /ligand_id="ChEBI:CHEBI:57288" FT Condition: F-[YF]-x(5)-[QR]-[RK]-x-G-x-G-x FT BINDING 160..169 FT /ligand="acetyl-CoA" FT /ligand_id="ChEBI:CHEBI:57288" FT Condition: S-x-K-[FLM]-x(2)-F-[LM]-x-K FT SITE 58 FT /note="Crucial for catalytic activity" FT Condition: Q case FT MOD_RES 56 FT /note="N6-acetyllysine; by autocatalysis" FT Condition: K FT MOD_RES 146 FT /note="N6-acetyllysine; by autocatalysis" FT Condition: K FT MOD_RES 233 FT /note="N6-acetyllysine; by autocatalysis" FT Condition: K FT MOD_RES 244 FT /note="N6-acetyllysine; by autocatalysis" FT Condition: K end case XX Size: 179-483; Related: None; Template: Q5SQI0; Q8K341; Q6PH17; Q22XZ3; O45100; Scope: Eukaryota Fusion: Nter: None Cter: None Duplicate: in CAEBR, CAEEL, DROME, TRYCR Plasmid: None Comments: None XX # Revision 1.14 2022/11/19 //