AC   MF_03137;
DC   Protein; auto
c?   <OC:Eukaryota>
TR   HAMAP; MF_00071; -; 1; level=0
XX
Names: GUF1
XX
ID   GUF1
case <OC:Vertebrata> or <OC:Fungi>
DE   RecName: Full=Translation factor <gene_name>, mitochondrial;
DE            EC=3.6.5.n1;
DE   AltName: Full=Elongation factor 4 homolog;
DE            Short=EF-4;
DE   AltName: Full=GTPase <gene_name>;
DE   AltName: Full=Ribosomal back-translocase;
else case <OC:Drosophilidae>
DE   RecName: Full=Translation factor waclaw, mitochondrial;
DE            EC=3.6.5.n1;
DE   AltName: Full=Elongation factor 4 homolog;
DE            Short=EF-4;
DE   AltName: Full=GTPase GUF1 homolog;
DE   AltName: Full=Ribosomal back-translocase;
else
DE   RecName: Full=Translation factor GUF1 homolog, mitochondrial;
DE            EC=3.6.5.n1;
DE   AltName: Full=Elongation factor 4 homolog;
DE            Short=EF-4;
DE   AltName: Full=GTPase GUF1 homolog;
DE   AltName: Full=Ribosomal back-translocase;
end case
case <OC:Vertebrata> or <OC:Fungi>
GN   Name=GUF1;
else case <OC:Drosophilidae>
GN   Name=waw;
end case
XX
CC   -!- FUNCTION: Promotes mitochondrial protein synthesis. May act as a
CC       fidelity factor of the translation reaction, by catalyzing a one-codon
CC       backward translocation of tRNAs on improperly translocated ribosomes.
CC       Binds to mitochondrial ribosomes in a GTP-dependent manner.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.n1;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane
CC       protein; Matrix side.
case not <AnyFeature:TransitM>
CC   -!- MISCELLANEOUS: This protein may be expected to contain an N-terminal
CC       transit peptide but none has been predicted.
end case
CC   -!- SIMILARITY: Belongs to the GTP-binding elongation factor family. LepA
CC       subfamily.
XX
DR   Pfam; PF00679; EFG_C; 1; trigger=no
DR   Pfam; PF00009; GTP_EFTU; 1; trigger=no
DR   Pfam; PF03144; GTP_EFTU_D2; 1; trigger=no
DR   Pfam; PF06421; LepA_C; 1; trigger=no
DR   PRINTS; PR00315; ELONGATNFCT; 1; trigger=no
DR   NCBIfam; TIGR01393; lepA; 1; trigger=no
DR   NCBIfam; TIGR00231; Small_GTP; 1; trigger=no
DR   PROSITE; PS00301; G_TR_1; 1; trigger=no
DR   General; TransitM; -; 0-1; trigger=yes
XX
KW   GTP-binding
KW   Hydrolase
KW   Membrane
KW   Mitochondrion
KW   Mitochondrion inner membrane
KW   Nucleotide-binding
KW   Protein biosynthesis
XX
GO   GO:0005759; C:mitochondrial matrix
GO   GO:0005525; F:GTP binding
GO   GO:0003924; F:GTPase activity
GO   GO:0043022; F:ribosome binding
GO   GO:0045727; P:positive regulation of translation
XX
FT   From: GUF1_YEAST (P46943)
FT   BINDING         53..60
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [AS]-H-[IV]-D-[HS]-G-K-[ST]
FT   BINDING         120..124
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: D-T-[CP]-G-H
FT   BINDING         174..177
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT   Condition: [NT]-K-[IMV]-D
XX
Size: 627-1085;
Related: MF_03138!;
Template: P46943; Q8N442;
Scope:
 Eukaryota
Fusion:
 Nter: None
 Cter: None
Duplicate: in TRYCR
Plasmid: None
Comments: None
XX
# Revision 1.11 2023/06/01
//