AC   MF_03152;
DC   Protein; auto
TR   HAMAP; MF_03152; -; 1; level=0
XX
Names: TRM5
XX
ID   TRM5
case <OC:Saccharomyces>
DE   RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
DE            EC=2.1.1.228;
DE   AltName: Full=M1G-methyltransferase;
DE   AltName: Full=tRNA [GM37] methyltransferase;
DE   AltName: Full=tRNA methyltransferase 5;
DE   Flags: Precursor;
else case <OC:Fungi>
DE   RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
DE            EC=2.1.1.228;
DE   AltName: Full=M1G-methyltransferase;
DE   AltName: Full=tRNA [GM37] methyltransferase;
DE   AltName: Full=tRNA methyltransferase 5;
else
DE   RecName: Full=tRNA (guanine(37)-N1)-methyltransferase;
DE            EC=2.1.1.228;
DE   AltName: Full=M1G-methyltransferase;
DE   AltName: Full=tRNA [GM37] methyltransferase;
DE   AltName: Full=tRNA methyltransferase 5 homolog;
end case
case <OC:Vertebrata>
GN   Name=TRMT5; Synonyms=TRM5;
else case <OC:Fungi>
GN   Name=TRM5;
end case
XX
case <OC:Saccharomyces>
CC   -!- FUNCTION: Specifically methylates the N1 position of guanosine-37 in
CC       various cytoplasmic and mitochondrial tRNAs. Methylation is not
CC       dependent on the nature of the nucleoside 5' of the target nucleoside.
CC       This is the first step in the biosynthesis of wybutosine (yW), a
CC       modified base adjacent to the anticodon of tRNAs and required for
CC       accurate decoding. Postspliced cytoplasmic tRNAs are imported into the
CC       nucleus, where this first step seems to take place, after which they
CC       are reexported to the cytoplasm, where the yW sythesis is completed by
CC       cytoplasmic enzymes.
else
CC   -!- FUNCTION: Specifically methylates the N1 position of guanosine-37 in
CC       various cytoplasmic and mitochondrial tRNAs. Methylation is not
CC       dependent on the nature of the nucleoside 5' of the target nucleoside.
CC       This is the first step in the biosynthesis of wybutosine (yW), a
CC       modified base adjacent to the anticodon of tRNAs and required for
CC       accurate decoding.
end case
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC         methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:36899, Rhea:RHEA-COMP:10145, Rhea:RHEA-COMP:10147,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.228;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix. Nucleus. Cytoplasm.
CC       Note=Predominantly in the mitochondria and in the nucleus.
CC   -!- SIMILARITY: Belongs to the TRM5 / TYW2 family.
XX
DR   PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1; trigger=no
DR   Pfam; PF02475; Met_10; 1; trigger=no
case not <OC:Saccharomyces>
DR   General; TransitM; -; 0-1; trigger=yes
end case
XX
KW   Cytoplasm
KW   Methyltransferase
KW   Mitochondrion
KW   Nucleus
KW   S-adenosyl-L-methionine
KW   Transferase
KW   tRNA processing
case <OC:Saccharomyces>
KW   Transit peptide
end case
XX
GO   GO:0005759; C:mitochondrial matrix
GO   GO:0005634; C:nucleus
GO   GO:0005737; C:cytoplasm
GO   GO:0030488; P:tRNA methylation
GO   GO:0052906; F:tRNA (guanine(37)-N1)-methyltransferase activity
XX
FT   From: TRM5_YEAST (P38793)
case <OC:Saccharomyces>
FT   TRANSIT         Nter..44
FT                   /note="Mitochondrion"
FT   CHAIN           45..Cter
FT                   /note="<name>"
end case
FT   BINDING         307..308
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: [DE]-[ILM]
FT   BINDING         335..336
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: [DE]-[AGNSV]
FT   BINDING         268
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: [HKR]
FT   BINDING         399
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT   Condition: N
XX
Size: 360-781;
Related: None;
Template: P38793; Q32P41;
Scope:
 Eukaryota
Fusion:
 Nter: None
 Cter: None
Duplicate: in ARATH, PHATC, TRYCR, VITVI
Plasmid: None
Comments: None
XX
# Revision 1.12 2023/10/13
//