AC   MF_03158;
DC   Protein; auto
TR   HAMAP; MF_03158; -; 1; level=0
XX
Names: THI4
XX
ID   THI4
case <OC:Viridiplantae>
DE   RecName: Full=Thiamine thiazole synthase, chloroplastic;
DE   AltName: Full=Thiazole biosynthetic enzyme;
DE            EC=2.4.2.60;
else
DE   RecName: Full=Thiamine thiazole synthase;
DE   AltName: Full=Thiazole biosynthetic enzyme;
DE            EC=2.4.2.60;
end case
case <OC:Saccharomycotina>
GN   Name=THI4;
else case <OC:Trichocomaceae>
GN   Name=thiA;
else case <OC:Viridiplantae>
GN   Name=THI1;
end case
XX
CC   -!- FUNCTION: Involved in biosynthesis of the thiamine precursor thiazole.
CC       Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-
CC       (2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an
CC       adenylated thiazole intermediate. The reaction includes an iron-
CC       dependent sulfide transfer from a conserved cysteine residue of the
CC       protein to a thiazole intermediate. The enzyme can only undergo a
CC       single turnover, which suggests it is a suicide enzyme. May have
CC       additional roles in adaptation to various stress conditions and in DNA
CC       damage tolerance.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-
CC         thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-
CC         carboxylate + 2 H(+) + 3 H2O + nicotinamide; Xref=Rhea:RHEA:55708,
CC         Rhea:RHEA-COMP:14264, Rhea:RHEA-COMP:14265, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17154, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57540, ChEBI:CHEBI:90873,
CC         ChEBI:CHEBI:139151; EC=2.4.2.60;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC       Note=Binds 1 Fe cation per subunit.;
CC   -!- SUBUNIT: Homooctamer.
case <OC:Viridiplantae>
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
else
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
end case
CC   -!- PTM: During the catalytic reaction, a sulfide is transferred from
CC       #{Cys-205} to a reaction intermediate, generating a dehydroalanine
CC       residue.
case <OC:Viridiplantae> and not <AnyFeature:TransitC>
CC   -!- MISCELLANEOUS: This protein may be expected to contain an N-terminal
CC       transit peptide but none has been predicted.
end case
CC   -!- SIMILARITY: Belongs to the THI4 family.
XX
DR   PRINTS; PR00368; FADPNR; 1; trigger=no
DR   NCBIfam; TIGR00292; TIGR00292; 1; trigger=no
case <OC:Viridiplantae>
DR   General; TransitC; -; 0-1; trigger=yes
end case
XX
case <OC:Viridiplantae>
KW   Chloroplast
KW   Plastid
else
KW   Cytoplasm
KW   Nucleus
end case
KW   Iron
KW   Metal-binding
KW   NAD
KW   Thiamine biosynthesis
KW   Transferase
XX
GO   GO:0005506; F:iron ion binding
GO   GO:0016763; F:pentosyltransferase activity
GO   GO:0005829; C:cytosol
case <OC:Viridiplantae>
GO   GO:0009570; C:chloroplast stroma
end case
GO   GO:0009228; P:thiamine biosynthetic process
GO   GO:0052837; P:thiazole biosynthetic process
XX
FT   From: THI4_YEAST (P32318)
FT   BINDING         97..98
FT                   /ligand="substrate"
FT   Condition: E-x
FT   BINDING         301..303
FT                   /ligand="substrate"
FT   Condition: R-M-x
FT   BINDING         76
FT                   /ligand="substrate"
FT   BINDING         105
FT                   /ligand="substrate"
FT   BINDING         170
FT                   /ligand="substrate"
FT   BINDING         207
FT                   /ligand="substrate"
FT   Condition: D
FT   BINDING         237
FT                   /ligand="substrate"
FT   Condition: H
FT   BINDING         291
FT                   /ligand="substrate"
FT   MOD_RES         205
FT                   /note="2,3-didehydroalanine (Cys)"
FT   Condition: C
XX
Size: 283-383;
Related: None;
Template: P32318; Q38814; Q1K6I4;
Scope:
 Eukaryota
Fusion:
 Nter: None
 Cter: None
Duplicate: in 9HYPO, MAIZE, ORYSJ, PHYPA, POPTR, PSEMZ, SELML, SORBI, SOYBN,
 VITVI, VOLCA
Plasmid: None
Comments: None
XX
# Revision 1.12 2023/06/01
//