AC MF_03176; DC Protein; auto TR HAMAP; MF_03176; -; 1; level=0 XX Names: PIF1 XX ID PIF1 DE RecName: Full=ATP-dependent DNA helicase PIF1; DE EC=3.6.4.12; DE AltName: Full=DNA repair and recombination helicase PIF1; GN Name=PIF1; XX case CC -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the CC maintenance of both mitochondrial and nuclear genome stability. CC Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA CC hybrids. Resolves G4 structures, preventing replication pausing and CC double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance CC of telomeric DNA. Inhibits telomere elongation, de novo telomere CC formation and telomere addition to DSBs via catalytic inhibition of CC telomerase. Reduces the processivity of telomerase by displacing active CC telomerase from DNA ends. Releases telomerase by unwinding the short CC telomerase RNA/telomeric DNA hybrid that is the intermediate in the CC telomerase reaction. Involved in the maintenance of ribosomal (rDNA). CC Required for efficient fork arrest at the replicaion fork barrier CC within rDNA. Involved in the maintenance of mitochondrial (mtDNA). CC Required to maintain mtDNA under conditions that introduce dsDNA breaks CC in mtDNA, either preventing or repairing dsDNA breaks. May inhibit CC replication progression to allow time for repair. May have a general CC role in chromosomal replication by affecting Okazaki fragment CC maturation. May have a role in conjunction with DNA2 helicase/nuclease CC in 5'-flap extension during Okazaki fragment processing. else case CC -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the CC maintenance of both mitochondrial and nuclear genome stability. CC Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA CC hybrids. Resolves G4 structures, preventing replication pausing and CC double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance CC of telomeric DNA. Inhibits telomere elongation, de novo telomere CC formation and telomere addition to DSBs via catalytic inhibition of CC telomerase. Reduces the processivity of telomerase by displacing active CC telomerase from DNA ends. Releases telomerase by unwinding the short CC telomerase RNA/telomeric DNA hybrid that is the intermediate in the CC telomerase reaction. Possesses an intrinsic strand annealing activity. else CC -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the CC maintenance of both mitochondrial and nuclear genome stability. end case CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; case or CC -!- SUBUNIT: Monomer. Interacts with telomerase. else CC -!- SUBUNIT: Monomer. end case CC -!- SUBCELLULAR LOCATION: Nucleus. Mitochondrion. CC -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily. XX DR Pfam; PF05970; PIF1; 1-2; trigger=no XX KW ATP-binding KW DNA damage KW DNA recombination KW DNA repair KW DNA-binding KW Helicase KW Hydrolase KW Mitochondrion KW Nucleotide-binding KW Nucleus XX case or GO GO:0051880; F:G-quadruplex DNA binding GO GO:0010521; F:telomerase inhibitor activity GO GO:0032211; P:negative regulation of telomere maintenance via telomerase end case GO GO:0005739; C:mitochondrion GO GO:0005634; C:nucleus GO GO:0005524; F:ATP binding GO GO:0043139; F:5'-3' DNA helicase activity GO GO:0006310; P:DNA recombination GO GO:0006281; P:DNA repair GO GO:0000002; P:mitochondrial genome maintenance XX FT From: PIF1_YEAST (P07271) FT BINDING 258..265 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT Condition: G-x-A-G-T-G-K-S FT DNA_BIND 727..746 FT Condition: [QH]-x-Y-V-A-[LI]-S-R-[AV]-x(5)-[LIV]-[QERH]-[LIV]-x(2)-F XX Size: 593-1113; Related: MF_03177!; Template: P07271; Q9H611; Q9UUA2; Scope: Eukaryota Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.10 2022/11/19 //