AC   MF_03211;
DC   Protein; auto
TR   HAMAP; MF_03211; -; 1; level=0
XX
Names: RNA_acetyltr_Nat10
XX
ID   NAT10
DE   RecName: Full=RNA cytidine acetyltransferase;
DE            EC=2.3.1.-;
DE   AltName: Full=18S rRNA cytosine acetyltransferase;
case <OC:Mammalia>
DE   + AltName: Full=N-acetyltransferase 10;
end case
case <OC:Vertebrata> or <OC:Fungi>
GN   Name=NAT10;
else case <OC:Caenorhabditis>
GN   Name=nath-10;
end case
XX
case <OC:Vertebrata>
CC   -!- FUNCTION: RNA cytidine acetyltransferase with specificity toward both
CC       18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine
CC       (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor
CC       rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the
CC       formation of ac4C in serine and leucine tRNAs. Requires the tRNA-
CC       binding adapter protein @gn(THUMPD1) for full tRNA acetyltransferase
CC       activity but not for 18S rRNA acetylation.
else case <OC:Fungi>
CC   -!- FUNCTION: RNA cytidine acetyltransferase with specificity toward both
CC       18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine
CC       (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor
CC       rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the
CC       formation of ac4C in serine and leucine tRNAs. Requires the tRNA-
CC       binding adapter protein @gn(TAN1) for full tRNA acetyltransferase
CC       activity but not for 18S rRNA acetylation.
else
CC   -!- FUNCTION: RNA cytidine acetyltransferase with specificity toward both
CC       18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine
CC       (ac4C) in 18S rRNA. Required for early nucleolar cleavages of precursor
CC       rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the
CC       formation of ac4C in serine and leucine tRNAs. Requires a tRNA-binding
CC       adapter protein for full tRNA acetyltransferase activity but not for
CC       18S rRNA acetylation.
end case
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a cytidine in 18S rRNA + acetyl-CoA + ATP + H2O = ADP + an
CC         N(4)-acetylcytidine in 18S rRNA + CoA + H(+) + phosphate;
CC         Xref=Rhea:RHEA:51424, Rhea:RHEA-COMP:13575, Rhea:RHEA-COMP:13576,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:74900, ChEBI:CHEBI:82748, ChEBI:CHEBI:456216;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a cytidine in tRNA + acetyl-CoA + ATP + H2O = ADP + an N(4)-
CC         acetylcytidine in tRNA + CoA + H(+) + phosphate;
CC         Xref=Rhea:RHEA:53876, Rhea:RHEA-COMP:13670, Rhea:RHEA-COMP:13671,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288,
CC         ChEBI:CHEBI:74900, ChEBI:CHEBI:82748, ChEBI:CHEBI:456216;
case <OC:Vertebrata>
CC   -!- SUBUNIT: Interacts with @gn(THUMPD1).
else case <OC:Fungi>
CC   -!- SUBUNIT: Interacts with @gn(TAN1).
end case
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus.
CC   -!- SIMILARITY: Belongs to the RNA cytidine acetyltransferase family. NAT10
CC       subfamily.
XX
DR   PROSITE; PS51186; GNAT; 1; trigger=yes
DR   Pfam; PF08351; DUF1726; 1; trigger=no
DR   Pfam; PF13718; GNAT_acetyltr_2; 1; trigger=no
DR   Pfam; PF05127; Helicase_RecD; 1; trigger=no
DR   Pfam; PF13725; tRNA_bind_2; 1; trigger=no
XX
KW   Acyltransferase
KW   ATP-binding
KW   Nucleotide-binding
KW   Nucleus
KW   rRNA processing
KW   Transferase
KW   tRNA processing
XX
GO   GO:0005730; C:nucleolus
GO   GO:0005524; F:ATP binding
GO   GO:0008080; F:N-acetyltransferase activity
GO   GO:0051391; P:tRNA acetylation
GO   GO:0000154; P:rRNA modification
GO   GO:0042274; P:ribosomal small subunit biogenesis
XX
FT   From: NAT10_YEAST (P53914)
FT   BINDING         286..295
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: G-R-G-K-S-A-[TAS]-[IVLM]-G-[IVLM]
FT   BINDING         638..640
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   Optional; Condition: [IV]-A-[ATV]
FT   BINDING         645..651
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   Optional; Condition: x(2)-[MLA]-[GN]-Y-G-x
FT   BINDING         475
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT   Optional; Condition: R
FT   BINDING         739
FT                   /ligand="acetyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57288"
FT   Optional; Condition: [RKN]
XX
Size: 825-1233;
Related: None;
Template: P53914; P87115; Q9H0A0;
Scope:
 Eukaryota
Fusion:
 Nter: None
 Cter: None
Duplicate: in ARATH, SOYBN
Plasmid: None
Comments: None
XX
# Revision 1.10 2022/11/19
//