AC MF_03231; DC Protein; auto TR HAMAP; MF_03231; -; 1; level=0 XX Names: SCS3 XX ID SCS3 case DE RecName: Full=Acyl-coenzyme A diphosphatase SCS3; DE EC=3.6.1.-; DE AltName: Full=FIT family protein SCS3; else DE RecName: Full=Acyl-coenzyme A diphosphatase SCS3 homolog; DE AltName: Full=FIT family protein SCS3 homolog; end case GN Name=SCS3; Synonyms=FIT2B; XX case CC -!- FUNCTION: Fatty acyl-coenzyme A (CoA) diphosphatase that hydrolyzes CC fatty acyl-CoA to yield acyl-4'-phosphopantetheine and adenosine 3',5'- CC bisphosphate. Preferentially hydrolyzes unsaturated long-chain acyl-CoA CC substrates in the endoplasmic reticulum (ER) lumen. This catalytic CC activity is required for maintaining ER structure and for lipid CC droplets (LDs) biogenesis, which are lipid storage organelles involved CC in maintaining lipid and energy homeostasis. May directly bind to CC diacylglycerol (DAGs) and triacylglycerol, which is also important for CC LD biogenesis. May support directional budding of nacent LDs from the CC ER into the cytosol by reducing DAG levels at sites of LD formation. CC May play a role in the regulation of cell morphology and cytoskeletal CC organization. Involved in phospholipid biosynthesis. CC -!- CATALYTIC ACTIVITY: CC Reaction=an acyl-CoA + H2O = adenosine 3',5'-bisphosphate + an acyl-4'- CC phosphopantetheine + 2 H(+); Xref=Rhea:RHEA:50044, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58342, ChEBI:CHEBI:58343, CC ChEBI:CHEBI:132023; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50045; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + H2O = adenosine 3',5'-bisphosphate + 2 CC H(+) + S-(9Z-octadecenoyl)-4'-phosphopantetheine; CC Xref=Rhea:RHEA:65564, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57387, ChEBI:CHEBI:58343, ChEBI:CHEBI:156553; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65565; CC -!- CATALYTIC ACTIVITY: CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = adenosine 3',5'- CC bisphosphate + 2 H(+) + S-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-4'- CC phosphopantetheine; Xref=Rhea:RHEA:65568, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57368, ChEBI:CHEBI:58343, CC ChEBI:CHEBI:156554; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:65569; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + hexadecanoyl-CoA = adenosine 3',5'-bisphosphate + 2 H(+) CC + S-hexadecanoyl-4'-phosphopantetheine; Xref=Rhea:RHEA:50032, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57379, CC ChEBI:CHEBI:58343, ChEBI:CHEBI:132018; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50033; else CC -!- FUNCTION: May hydrolyze fatty acyl-CoA to yield acyl-4'- CC phosphopantetheine and adenosine 3',5'-bisphosphate. May be required CC for maintaining ER structure and for lipid droplets (LDs) biogenesis, CC which are lipid storage organelles involved in maintaining lipid and CC energy homeostasis. end case CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass CC membrane protein. CC -!- SIMILARITY: Belongs to the FIT family. Fungal FIT2B/SCS3 subfamily. XX DR Pfam; PF10261; Scs3p; 1-2; trigger=no DR General; Signal; -; 0-1; trigger=no DR General; Transmembrane; -; 0-7; trigger=no XX case KW Hydrolase KW Lipid biosynthesis KW Phospholipid biosynthesis KW Phospholipid metabolism end case KW Endoplasmic reticulum KW Membrane KW Transmembrane KW Transmembrane helix XX case GO GO:0016818; F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides end case GO GO:0005789; C:endoplasmic reticulum membrane GO GO:0140042; P:lipid droplet formation XX FT From: SCS3_YEAST (P53012) FT ACT_SITE 235 FT Group: 1; Condition: H FT ACT_SITE 350 FT Group: 1; Condition: H XX Size: 246-397; Related: None; Template: P53012; Scope: Eukaryota; Ascomycota Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.3 2021/05/20 //