AC   MF_04001;
DC   Protein; auto
TR   HAMAP; MF_04001; -; 1; level=0
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Names: PPV_E2
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ID   VE2
DE   RecName: Full=Regulatory protein E2;
GN   Name=E2;
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CC   -!- FUNCTION: Plays a role in the initiation of viral DNA replication. A
CC       dimer of E2 interacts with a dimer of E1 in order to improve
CC       specificity of E1 DNA binding activity. Once the complex recognizes and
CC       binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also
CC       regulates viral transcription through binding to the E2RE response
CC       element (5'-ACCNNNNNNGGT-3') present in multiple copies in the
CC       regulatory regions of the viral genome. Activates or represses
CC       transcription depending on E2RE's position with regards to proximal
CC       promoter elements including the TATA-box. Repression occurs by
CC       sterically hindering the assembly of the transcription initiation
CC       complex.
CC   -!- SUBUNIT: Binds DNA as homodimer. Interacts with protein E1; this
CC       interaction greatly increases E1 DNA-binding activity. Interacts with
CC       protein L1; this interaction enhances E2-dependent replication and
CC       transcription activation. Interacts with protein L2; this interaction
CC       inhibits E2 transcriptional activity but not DNA replication function
CC       E2. Interacts with protein E7; this interaction inhibits E7 oncogenic
CC       activity. Interacts with host TAF1; this interaction modulates E2-
CC       dependent transcriptional regulation. Interacts with host BRD4; this
CC       interaction mediates E2 transcriptional activation function.
CC       Additionally, the interaction with host BRD4 on mitotic chromosomes
CC       mediates tethering of the viral genome. Interacts with host TOPBP1;
CC       this interaction is required for optimal viral DNA replication.
CC   -!- SUBCELLULAR LOCATION: Host nucleus.
CC   -!- PTM: Phosphorylated.
case <FTTag:Ubl>
CC   -!- PTM: Sumoylation plays a regulatory role in E2 transcriptional
CC       activity.
end case
CC   -!- SIMILARITY: Belongs to the papillomaviridae E2 protein family.
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DR   Pfam; PF00511; PPV_E2_C; 1; trigger=no
DR   Pfam; PF00508; PPV_E2_N; 1; trigger=no
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KW   Activator
KW   DNA replication
KW   DNA-binding
KW   Early protein
KW   Host nucleus
KW   Phosphoprotein
KW   Repressor
KW   Transcription
KW   Transcription regulation
case <FTTag:Ubl>
KW   Ubl conjugation
KW   Isopeptide bond
end case
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GO   GO:0042025; C:host cell nucleus
GO   GO:0003677; F:DNA binding
GO   GO:0000166; F:nucleotide binding
GO   GO:0003700; F:DNA-binding transcription factor activity
GO   GO:0006275; P:regulation of DNA replication
GO   GO:0006351; P:DNA-templated transcription
GO   GO:0039693; P:viral DNA genome replication
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FT   From: VE2_HPV16 (P03120)
FT   REGION          1..200
FT                   /note="Transactivation domain"
FT   REGION          285..Cter
FT                   /note="DNA-binding domain"
FT   CROSSLNK        292
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO)"
FT   Tag: Ubl; Condition: K
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Size: 310-514;
Related: None;
Template: P03120;
Scope:
 Viruses; Papillomaviridae
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
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# Revision 1.7 2022/06/28
//