AC MF_04044; DC Protein; auto TR HAMAP; MF_04044; -; 1; level=0 XX Names: HSV_LTP XX ID LTP DE RecName: Full=Large tegument protein deneddylase; DE EC=3.4.19.12; DE EC=3.4.22.-; XX CC -!- FUNCTION: Large tegument protein that plays multiple roles in the viral CC cycle. During viral entry, remains associated with the capsid while CC most of the tegument is detached and participates in the capsid CC transport toward the host nucleus. Plays a role in the routing of the CC capsid at the nuclear pore complex and subsequent uncoating. Within the CC host nucleus, acts as a deneddylase and promotes the degradation of CC nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes CC nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These CC modifications prevent host cell cycle S-phase progression and create a CC favorable environment allowing efficient viral genome replication. CC Participates later in the secondary envelopment of capsids. Indeed, CC plays a linker role for the association of the outer viral tegument to CC the capsids together with the inner tegument protein. CC -!- CATALYTIC ACTIVITY: CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- CC residue protein attached to proteins as an intracellular targeting CC signal).; EC=3.4.19.12; CC -!- SUBUNIT: Interacts with host CUL1 and CUL4A; these interactions inhibit CC the E3 ligase activity of cullins. Interacts with inner tegument CC protein. Interacts with capsid vertex specific component CVC2. CC Interacts with the major capsid protein/MCP. CC -!- SUBCELLULAR LOCATION: Virion tegument. Host cytoplasm. Host nucleus. CC Note=Tightly associated with the capsid. XX DR PROSITE; PS51521; HTUSP; 1; trigger=yes DR Pfam; PF04843; Herpes_teg_N; 1; trigger=no DR Pfam; PF03586; Herpes_UL36; 1; trigger=no XX KW Host cytoplasm KW Host nucleus KW Host-virus interaction KW Hydrolase KW Modulation of host ubiquitin pathway by viral deubiquitinase KW Modulation of host ubiquitin pathway by virus KW Protease KW Repeat KW Thiol protease KW Ubl conjugation pathway KW Virion KW Virion tegument XX GO GO:0030430; C:host cell cytoplasm GO GO:0042025; C:host cell nucleus GO GO:0019033; C:viral tegument GO GO:0004843; F:cysteine-type deubiquitinase activity GO GO:0039648; P:modulation by symbiont of host protein ubiquitination XX FT From: LTP_HHV11 (P10220) FT REGION Nter..273 FT /note="Deubiquitination activity" FT REGION 579..609 FT /note="Interaction with inner tegument protein" FT ACT_SITE 65 FT Condition: C FT ACT_SITE 197 FT Condition: D FT ACT_SITE 199 FT Condition: H XX Size: 2059-3755; Related: None; Template: P10220; P03186; Scope: Viruses; Herpesviridae Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.8 2023/02/09 //