AC MF_04065; DC Protein; auto TR HAMAP; MF_04065; -; 1; level=0 XX Names: INFV_RDRP XX ID RDRP DE RecName: Full=RNA-directed RNA polymerase catalytic subunit; DE EC=2.7.7.48; DE AltName: Full=Polymerase basic protein 1; DE Short=PB1; DE AltName: Full=RNA-directed RNA polymerase subunit P1; GN Name=PB1; XX CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for CC replication and transcription of virus RNA segments. The transcription CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides CC by PA. In turn, these short capped RNAs are used as primers by PB1 for CC transcription of viral mRNAs. During virus replication, PB1 initiates CC RNA synthesis and copies vRNA into complementary RNA (cRNA) which in CC turn serves as a template for the production of more vRNAs. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; CC EC=2.7.7.48; CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). CC Interacts (via C-terminus) with PB2 (via N-terminus); this interaction CC is essential for transcription initiation. CC -!- SUBCELLULAR LOCATION: Host nucleus. Host cytoplasm. CC -!- PTM: Phosphorylated by host PRKCA. CC -!- SIMILARITY: Belongs to the influenza viruses polymerase PB1 family. XX DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1; trigger=no DR Pfam; PF00602; Flu_PB1; 1; trigger=no DR PIRSF; PIRSF000827; RdRPol_OMV; 1; trigger=no XX KW Eukaryotic host gene expression shutoff by virus KW Eukaryotic host transcription shutoff by virus KW Host cytoplasm KW Host gene expression shutoff by virus KW Host nucleus KW Host-virus interaction KW Inhibition of host RNA polymerase II by virus KW Nucleotide-binding KW Nucleotidyltransferase KW Phosphoprotein KW RNA-directed RNA polymerase KW Transferase KW Viral RNA replication KW Viral transcription XX GO GO:0030430; C:host cell cytoplasm GO GO:0042025; C:host cell nucleus GO GO:0000166; F:nucleotide binding GO GO:0003968; F:RNA-dependent RNA polymerase activity GO GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity GO GO:0006351; P:DNA-templated transcription GO GO:0039694; P:viral RNA genome replication XX FT From: RDRP_I34A1 (P03431) FT DOMAIN 286..483 FT /note="RdRp catalytic" FT REGION 249..256 FT /note="Promoter-binding site" FT MOTIF 187..195 FT /note="Nuclear localization signal" FT MOTIF 203..216 FT /note="Nuclear localization signal" XX Size: 751-758; Related: None; Template: P03431; Scope: Viruses; Orthomyxoviridae Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.9 2022/09/29 //