AC   MF_04085;
DC   Protein; auto
TR   HAMAP; MF_04085; -; 1; level=0
XX
Names: ARENA_NCAP
XX
ID   NCAP
DE   RecName: Full=Nucleoprotein;
DE            EC=3.1.13.-;
DE   AltName: Full=Nucleocapsid protein;
DE   AltName: Full=Protein N;
GN   Name=N;
XX
CC   -!- FUNCTION: Encapsidates the genome, protecting it from nucleases. The
CC       encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as
CC       template for viral transcription and replication. The increased
CC       presence of protein N in host cell does not seem to trigger the switch
CC       from transcription to replication as observed in other negative strain
CC       RNA viruses. Through the interaction with host IKBKE, strongly inhibits
CC       the phosphorylation and nuclear translocation of host IRF3, a protein
CC       involved in interferon activation pathway, leading to the inhibition of
CC       interferon-beta and IRF3-dependent promoters activation. Encodes also a
CC       functional 3'-5' exoribonuclease that degrades preferentially dsRNA
CC       substrates and therby participates in the suppression of interferon
CC       induction.
CC   -!- SUBUNIT: Homomultimerizes to form the nucleocapsid. Binds to viral
CC       genomic RNA. Interacts with glycoprotein G2. Interacts with protein Z;
CC       this interaction probably directs the encapsidated genome to budding
CC       sites. Interacts with protein L; this interaction does not interfere
CC       with Z-L interaction. Interacts with host IKBKE (via Protein kinase
CC       domain); the interaction inhibits IKBKE kinase activity.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm.
CC   -!- DOMAIN: The N-terminal region is important for the cap-binding activity
CC       while the C-terminal region contains the 3'-5' exoribonuclease
CC       activity. A CCHE zinc binding site is present in the C-terminal region
CC       and may thus contribute to the substrate binding and/or the specificity
CC       of the exonuclease activity.
CC   -!- SIMILARITY: Belongs to the arenaviridae nucleocapsid protein family.
XX
DR   Pfam; PF17290; Arena_ncap_C; 1; trigger=no
DR   Pfam; PF00843; Arena_nucleocap; 1; trigger=no
DR   PIRSF; PIRSF004029; N_ArenaV; 1; trigger=no
XX
KW   Capsid protein
KW   Helical capsid protein
KW   Host cytoplasm
KW   Host-virus interaction
KW   Hydrolase
KW   Inhibition of host IKBKE by virus
KW   Inhibition of host innate immune response by virus
KW   Inhibition of host RLR pathway by virus
KW   Interferon antiviral system evasion
KW   Ribonucleoprotein
KW   RNA-binding
KW   Viral immunoevasion
KW   Viral nucleoprotein
KW   Virion
KW   Metal-binding
KW   Zinc
XX
GO   GO:0019029; C:helical viral capsid
GO   GO:0030430; C:host cell cytoplasm
GO   GO:0019013; C:viral nucleocapsid
GO   GO:0046872; F:metal ion binding
GO   GO:0003723; F:RNA binding
GO   GO:0039689; P:negative stranded viral RNA replication
GO   GO:0039696; P:RNA-templated viral transcription
GO   GO:0039724; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity
XX
FT   From: NCAP_LASSJ (P13699)
FT   REGION          54..241
FT                   /note="Binding site for the cap structure m7GTP"
FT   BINDING         389
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT   Condition: D
FT   BINDING         391
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT   Condition: E
FT   BINDING         399
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Condition: E
FT   BINDING         506
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Condition: C
FT   BINDING         509
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Condition: H
FT   BINDING         529
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Condition: C
FT   BINDING         533
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT   Condition: D
FT   SITE            466
FT                   /note="Important for exonuclease activity"
FT   Condition: D
XX
Size: 557-570;
Related: None;
Template: P09992; P14239; P13699;
Scope:
 Viruses; Arenaviridae
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.8 2022/11/19
//