AC MF_04111; DC Protein; auto TR HAMAP; MF_04111; -; 1; level=0 XX Names: ENDOLYSIN_T7 XX ID ENLYS DE RecName: Full=Endolysin; DE EC=3.5.1.28; DE AltName: Full=N-acetylmuramoyl-L-alanine amidase; XX CC -!- FUNCTION: Plays an important role in the switch between viral CC transcription and genome replication. Once produced in sufficient CC amount, interacts with and inhibits the viral RNA polymerase that CC becomes unable to produce additional late transcripts. This lysozyme- CC polymerase complex in turn plays an active role in viral genome CC replication and packaging. CC -!- FUNCTION: Endolysin with amidase activity that degrades host CC peptidoglycans and participates with the holin and spanin proteins in CC the sequential events which lead to the programmed host cell lysis CC releasing the mature viral particles. Once the holin has permeabilized CC the host cell membrane, the endolysin can reach the periplasm and CC breaking down the peptidoglycan layer. CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L- CC amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Note=Zn(2+) is required for amidase activity CC -!- ACTIVITY REGULATION: Binding to the viral RNA polymerase inhibits CC amidase activity. CC -!- SUBUNIT: Interacts with the viral RNA polymerase. CC -!- SUBCELLULAR LOCATION: Host cytoplasm. Note=The endolysin is CC cytoplasmic, but can reach the periplasmic space with the help of the CC holins which disrupt the host cell membrane. CC -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. XX DR Pfam; PF01510; Amidase_2; 1; trigger=no XX KW Antimicrobial KW Bacteriolytic enzyme KW Cytolysis KW Host cell lysis by virus KW Host cytoplasm KW Hydrolase KW Late protein KW Metal-binding KW Viral release from host cell KW Zinc XX GO GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity GO GO:0044659; P:viral release from host cell by cytolysis GO GO:0009253; P:peptidoglycan catabolic process XX FT From: ENLYS_BPT7 (P00806) FT BINDING 18 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT Condition: H FT BINDING 123 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT Condition: H FT BINDING 131 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT Condition: C FT SITE 47 FT /note="Essential for amidase activity and zinc hydrate FT coordination" FT Condition: Y XX Size: 146-153; Related: None; Template: P00806; Scope: Viruses; Caudoviricetes Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.10 2022/11/19 //