AC   MF_04121;
DC   Protein; auto
TR   HAMAP; MF_04121; -; 1; level=0
XX
Names: TRANSGLYCOSYLASE_T7
XX
ID   EXLYS
case <FTTag:transglycosylase>
DE   RecName: Full=Peptidoglycan transglycosylase gp16;
DE            EC=4.2.2.n1;
DE   AltName: Full=Internal core protein gp16;
else
DE   RecName: Full=Putative peptidoglycan transglycosylase gp16;
DE   AltName: Full=Internal core protein gp16;
end case
XX
case <FTTag:transglycosylase>
CC   -!- FUNCTION: Component of the cylindrical core that assembles on the inner
CC       surface of the capsid during capsid formation and plays a role in viral
CC       DNA ejection into the host cell. The inner core is composed of stacked
CC       rings of gp14, gp15 and gp16 proteins. Following binding to the host
CC       cell surface, the internal core is disassembled and gp16 is ejected
CC       along with gp14 and gp15 into the infected cell. Gp16 probably inserts
CC       in the host inner membrane and remains associated with gp15. The gp15-
CC       gp16 complex binds to both the viral DNA and the host inner membrane,
CC       probably escorting the leading end of the genome through the periplasm
CC       and controlling the extent of DNA translocated into the host cell.
CC       Functions as an exolysin that catalyzes the cleavage of the glycosidic
CC       bonds between N-acetylmuramic acid and N-acetylglucosamine residues in
CC       peptidoglycans allowing the local digestion of the bacterial
CC       peptidoglycan wall.
else
CC   -!- FUNCTION: Component of the cylindrical core that assembles on the inner
CC       surface of the capsid during capsid formation and plays a role in viral
CC       DNA ejection into the host cell. The inner core is composed of stacked
CC       rings of gp14, gp15 and gp16 proteins. Following binding to the host
CC       cell surface, the internal core is disassembled and gp16 is ejected
CC       along with gp14 and gp15 into the infected cell. Gp16 probably inserts
CC       in the host inner membrane and remains associated with gp15. The gp15-
CC       gp16 complex binds to both the viral DNA and the host inner membrane,
CC       probably escorting the leading end of the genome through the periplasm
CC       and controlling the extent of DNA translocated into the host cell.
end case
case <FTTag:transglycosylase>
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Exolytic cleavage of the (1->4)-beta-glycosidic linkage
CC         between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine
CC         (GlcNAc) residues in peptidoglycan, from either the reducing or the
CC         non-reducing ends of the peptidoglycan chains, with concomitant
CC         formation of a 1,6-anhydrobond in the MurNAc residue.; EC=4.2.2.n1;
end case
CC   -!- SUBUNIT: Homotetramer. Interacts with gp15; after ejection the gp15-
CC       gp16 complex composed of a gp15 octamer and a gp16 tetramer probably
CC       binds both the viral DNA and the host inner membrane.
CC   -!- SUBCELLULAR LOCATION: Virion. Host cell inner membrane; Single-pass
CC       membrane protein. Note=The gp15-gp16 complex spans the periplasm and
CC       the cytoplasmic membrane.
case <FTTag:transglycosylase>
CC   -!- DOMAIN: The N-terminus contains the transglycosylase activity. The C-
CC       terminus is essential for the viral DNA translocation into the host
CC       cytoplasm.
end case
CC   -!- SIMILARITY: Belongs to the transglycosylase Slt family.
XX
DR   PROSITE; PS00922; TRANSGLYCOSYLASE; 1; trigger=no
DR   Pfam; PF01464; SLT; 1; trigger=no
DR   General; Coiled_coil; -; 0-unlimited; trigger=yes
DR   General; Transmembrane; -; 0-1; trigger=no
XX
KW   Host cell inner membrane
KW   Host cell membrane
KW   Host membrane
case <FTTag:transglycosylase>
KW   Antimicrobial
KW   Bacteriolytic enzyme
KW   Degradation of host cell envelope components during virus entry
KW   Degradation of host peptidoglycans during virus entry
KW   Hydrolase
KW   Lyase
end case
KW   Membrane
KW   Transmembrane
KW   Transmembrane helix
KW   Viral genome ejection through host cell envelope
KW   Viral penetration into host cytoplasm
KW   Viral short tail ejection system
KW   Virion
KW   Virus entry into host cell
XX
GO   GO:0044423; C:virion component
GO   GO:0098932; P:disruption by virus of host cell wall peptidoglycan during virus entry
GO   GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism
case <FTTag:transglycosylase>
GO   GO:0008933; F:lytic transglycosylase activity
end case
XX
FT   From: EXLYS_BPT7 (P03726)
FT   TOPO_DOM        Nter..1136
FT                   /note="Periplasmic"
FT   TRANSMEM        1137..1157
FT                   /note="Helical"
FT   TOPO_DOM        1158..Cter
FT                   /note="Cytoplasmic"
FT   REGION          24..111
FT                   /note="Transglycosylase SLT-type domain"
FT   REGION          1314..1318
FT                   /note="Essential for viral DNA translocation"
FT   Condition: R-E-[KR]-[KR]-K
FT   ACT_SITE        37
FT   Tag: transglycosylase; Condition: E
XX
Size: 1250-1550;
Related: None;
Template: P03726;
Scope:
 Viruses; Caudoviricetes
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.6 2023/02/16
//