AC MF_04151; DC Protein; auto TR HAMAP; MF_04151; -; 1; level=0 XX Names: NEEDLE_T4 XX ID NEEDL case and DE RecName: Full=Pre-baseplate central spike protein Gp5; DE Short=Pre-Gp5; DE AltName: Full=Peptidoglycan hydrolase gp5; DE EC=3.2.1.17; DE Contains: DE RecName: Full=Baseplate central spike protein Gp5*; DE AltName: Full=Mature Gp5; DE Contains: DE RecName: Full=Gp5C; GN Name=5; else case and not DE RecName: Full=Pre-baseplate central spike protein; DE AltName: Full=Peptidoglycan hydrolase; DE EC=3.2.1.17; DE Contains: DE RecName: Full=Mature baseplate central spike protein; DE Contains: DE RecName: Full=Baseplate central spike protein C-terminus; else DE RecName: Full=Baseplate central spike protein; DE AltName: Full=Peptidoglycan hydrolase; DE EC=3.2.1.17; end case XX case and CC -!- FUNCTION: [Baseplate central spike protein Gp5*]: Baseplate central CC spike complex-associated lysozyme that is essential for the localized CC hydrolysis of bacterial cell wall, so that the tail tube, through which CC the phage DNA is ejected, can penetrate to the host inner membrane. The CC tail lysozyme complex at the tip of the tail tube penetrates through CC the outer membrane into the periplasm and during that process, gp5* CC dissociates from gp5C and activated. Due to the lower pH in the CC periplasm, gp5* would dissociate from gp27 which probably still binds CC to the tip of the tube. This way, lysozyme domain is released and CC locally digests the peptidoglycan layer to make a hole to let the tube CC penetrate to the inner membrane. Involved in the tail assembly. CC -!- CATALYTIC ACTIVITY: [Baseplate central spike protein Gp5*]: CC Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic CC acid and N-acetyl-D-glucosamine residues in a peptidoglycan and CC between N-acetyl-D-glucosamine residues in chitodextrins.; CC EC=3.2.1.17; CC -!- SUBUNIT: [Baseplate central spike protein Gp5*]: Monomer. The central CC spike complex, which creates an extension of the tail tube, is made up CC of three copies of the gp27-gp5*-gp5C complex and one copy of gp5.4. CC Part of the baseplate macromolecular complex which consists of gp5*, CC gp5C, gp5.4, gp27 (central spike complex); gp6, gp25, gp53 (inner CC baseplate); gp7, gp8 (intermediate baseplate); gp9, gp10, gp11, gp12 CC (peripheral); gp48 and gp54 (proximal region of the tail tube). CC -!- SUBUNIT: [Gp5C]: Homotrimer. The central spike complex, which creates CC an extension of the tail tube, is made up of three copies of the gp27- CC gp5*-gp5C complex and one copy of gp5.4. Part of the baseplate CC macromolecular complex which consists of gp5*, gp5C, gp5.4, gp27 CC (central spike complex); gp6, gp25, gp53 (inner baseplate); gp7, gp8 CC (intermediate baseplate); gp9, gp10, gp11, gp12 (peripheral); gp48 and CC gp54 (proximal region of the tail tube). CC -!- SUBUNIT: [Pre-baseplate central spike protein Gp5]: Homotrimer. CC -!- SUBCELLULAR LOCATION: [Baseplate central spike protein Gp5*]: Virion. CC Note=Present in 3 copies in the baseplate. CC -!- SUBCELLULAR LOCATION: [Gp5C]: Virion. Note=Present in 3 copies in the CC baseplate. CC -!- SUBCELLULAR LOCATION: [Pre-baseplate central spike protein Gp5]: CC Virion. Note=Present in the baseplate. CC -!- PTM: [Pre-baseplate central spike protein Gp5]: In the fully assembled CC virus, gp5 precursor is cleaved to form the mature tail lysozyme gp5*, CC and a C-terminus fragment, gp5C. The two fragments remain associated CC with the virion. The enzymatic activity of the precursor is about 10% CC of that of mature gp5*. CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 24 family. else case and not CC -!- FUNCTION: [Mature baseplate central spike protein]: Baseplate central CC spike complex-associated lysozyme that is essential for the localized CC hydrolysis of bacterial cell wall, so that the tail tube, through which CC the phage DNA is ejected, can penetrate to the host inner membrane. The CC tail lysozyme complex at the tip of the tail tube penetrates through CC the outer membrane into the periplasm. This way, lysozyme domain is CC released and locally digests the peptidoglycan layer to make a hole to CC let the tube penetrate to the inner membrane. Involved in the tail CC assembly. CC -!- CATALYTIC ACTIVITY: [Mature baseplate central spike protein]: CC Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic CC acid and N-acetyl-D-glucosamine residues in a peptidoglycan and CC between N-acetyl-D-glucosamine residues in chitodextrins.; CC EC=3.2.1.17; CC -!- SUBUNIT: [Mature baseplate central spike protein]: Monomer. The central CC spike complex creates an extension of the tail tube. CC -!- SUBUNIT: [Baseplate central spike protein C-terminus]: Homotrimer. The CC central spike complex creates an extension of the tail tube. CC -!- SUBUNIT: [Pre-baseplate central spike protein]: Homotrimer. CC -!- SUBCELLULAR LOCATION: [Mature baseplate central spike protein]: Virion. CC Note=Present in the baseplate. CC -!- SUBCELLULAR LOCATION: [Baseplate central spike protein C-terminus]: CC Virion. Note=Present in the baseplate. CC -!- SUBCELLULAR LOCATION: [Pre-baseplate central spike protein]: CC Virion. Note=Present in the baseplate. CC -!- PTM: [Pre-baseplate central spike protein]: In the fully assembled CC virus, the pre-baseplate central spike protein is cleaved to form the CC mature baseplate central spike protein, and a C-terminus fragment, the CC baseplate central spike protein C-terminus. The two fragments remain CC associated with the virion. CC -!- CAUTION: The cleavage site between the mature baseplate central spike CC protein and the baseplate central spike protein C-terminus is CC uncertain. CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 24 family. else CC -!- FUNCTION: [Baseplate central spike protein]: Baseplate central spike CC complex-associated lysozyme that is essential for the localized CC hydrolysis of bacterial cell wall, so that the tail tube, through which CC the phage DNA is ejected, can penetrate to the host inner membrane. The CC tail lysozyme complex at the tip of the tail tube penetrates through CC the outer membrane into the periplasm. This way, lysozyme domain is CC released and locally digests the peptidoglycan layer to make a hole to CC let the tube penetrate to the inner membrane. Involved in the tail CC assembly. CC -!- CATALYTIC ACTIVITY: [Baseplate central spike protein]: CC Reaction=Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic CC acid and N-acetyl-D-glucosamine residues in a peptidoglycan and CC between N-acetyl-D-glucosamine residues in chitodextrins.; CC EC=3.2.1.17; CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 24 family. end case XX DR Pfam; PF06715; Gp5_C; 3; trigger=no DR Pfam; PF06714; Gp5_OB; 1; trigger=no DR Pfam; PF00959; Phage_lysozyme; 1; trigger=no DR PRINTS; PR00684; T4LYSOZYME; 1; trigger=no XX KW Antimicrobial KW Bacteriolytic enzyme KW Degradation of host cell envelope components during virus entry KW Degradation of host peptidoglycans during virus entry KW Glycosidase KW Hydrolase KW Late protein KW Viral baseplate protein KW Viral genome ejection through host cell envelope KW Viral penetration into host cytoplasm KW Viral release from host cell KW Viral tail assembly KW Viral tail protein KW Virion KW Virus entry into host cell XX GO GO:0098015; C:virus tail GO GO:0098025; C:virus tail, baseplate GO GO:0003796; F:lysozyme activity GO GO:0098932; P:disruption by virus of host cell wall peptidoglycan during virus entry GO GO:0009253; P:peptidoglycan catabolic process GO GO:0098003; P:viral tail assembly XX FT From: NEEDL_BPT4 (P16009) FT ACT_SITE 184 FT /note="Proton donor" FT Condition: E FT ACT_SITE 193 FT /note="Nucleophile" FT Condition: D FT SITE 351..352 FT /note="Cleavage" FT Tag: Cleavage; Optional; Condition: S-A case and FT CHAIN Nter..Cter FT /note="Pre-baseplate central spike protein Gp5" FT CHAIN Nter..351 FT /note="Baseplate central spike protein Gp5*" FT CHAIN 352..Cter FT /note="Gp5C" else case and not FT CHAIN Nter..Cter FT /note="Pre-baseplate central spike protein" FT CHAIN Nter..351 FT /note="Mature baseplate central spike protein" FT CHAIN 352..Cter FT /note="Baseplate central spike protein C-terminus" else FT CHAIN Nter..Cter FT /note="Baseplate central spike protein" end case XX Size: 550-700; Related: None; Template: P16009; Scope: Viruses Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: None XX # Revision 1.3 2021/03/25 //