AC   MF_04157;
DC   Protein; auto
TR   HAMAP; MF_04157; -; 1; level=0
XX
Names: PRIMASE_T4
XX
ID   PRIM
DE   RecName: Full=DNA primase;
DE            EC=2.7.7.-;
DE   AltName: Full=DNA priming protein;
XX
CC   -!- FUNCTION: Synthesizes short RNA primers for the lagging strand DNA
CC       replication. The primase synthesizes short RNA primers on the lagging
CC       strand that the polymerase elongates using dNTPs. Recognizes two
CC       trinucleotide sequences 5'-GTT-3' and 5'-GCT-3' in vitro, but uses only
CC       the first as the priming site in vivo.
CC   -!- SUBUNIT: Monomer. Hexamer. Interacts with the DnaB-like replicative
CC       helicase; this interaction forms the active primosome complex, which is
CC       composed of 6 helicase and 1 primase subunits and expresses full
CC       helicase and primase activities. Interacts (via C-terminus) with the
CC       single-stranded DNA-binding protein. Part of the replicase complex that
CC       includes the DNA polymerase, the polymerase clamp, the clamp loader
CC       complex, the single-stranded DNA binding protein, the primase, the
CC       DnaB-like replicative helicase and the helicase assembly factor.
CC   -!- SIMILARITY: Belongs to the Tequatrovirus DNA primase family.
XX
DR   Pfam; PF08275; Toprim_N; 1; trigger=no
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KW   Viral DNA replication
KW   Early protein
KW   Transferase
case <FTGroup:1>
KW   Zinc
end case
XX
GO   GO:0039693; P:viral DNA genome replication
GO   GO:0016740; F:transferase activity
GO   GO:0003896; F:DNA primase activity
case <FTGroup:1>
GO   GO:0008270; F:zinc ion binding
end case
XX
FT   From: PRIM_BPT4 (P04520)
FT   BINDING         37
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
FT   BINDING         40
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
FT   BINDING         65
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
FT   BINDING         68
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT   Group: 1; Condition: C
XX
Size: 260-460;
Related: None;
Template: P04520;
Scope:
 Viruses; Enterobacteria phage T4
Fusion:
 Nter: None
 Cter: None
Duplicate: None
Plasmid: None
Comments: None
XX
# Revision 1.5 2022/11/19
//