HAMAP rule MF_00785
General rule information
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Accession | MF_00785 |
Dates | 24-MAY-2004 (Created)
19-NOV-2022 (Last updated, Version 29) |
Name | CbiX |
Scope(s) |
Archaea |
Template(s) | O29537 (CBIX_ARCFU); O27448 (CFBA_METTH); P61816 (CFBA_METBF); Q8TJZ5 (CFBA_METAC); [ Recover all ] |
Triggered by |
HAMAP; MF_00785 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
Identifier | CFBA |
Protein name | RecName: Full=Sirohydrochlorin cobaltochelatase; EC=4.99.1.3; AltName: Full=CbiXS; AltName: Full=Sirohydrochlorin nickelchelatase; EC=4.99.1.11; |
Gene name | Name=cbiX; Synonyms=cfbA; |
else | |
Identifier | CBIX |
Protein name | RecName: Full=Sirohydrochlorin cobaltochelatase; EC=4.99.1.3; AltName: Full=CbiXS; |
Gene name | Name=cbiX; |
end case |
Comments
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case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
FUNCTION | Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni- sirohydrochlorin. |
CATALYTIC ACTIVITY | Reaction=Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin; Xref=Rhea:RHEA:15893, ChEBI:CHEBI:15378, ChEBI:CHEBI:48828, ChEBI:CHEBI:58351, ChEBI:CHEBI:60049; EC=4.99.1.3; |
CATALYTIC ACTIVITY | Reaction=2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin; Xref=Rhea:RHEA:52796, ChEBI:CHEBI:15378, ChEBI:CHEBI:49786, ChEBI:CHEBI:58351, ChEBI:CHEBI:136841; EC=4.99.1.11; |
else | |
FUNCTION | Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. |
CATALYTIC ACTIVITY | Reaction=Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin; Xref=Rhea:RHEA:15893, ChEBI:CHEBI:15378, ChEBI:CHEBI:48828, ChEBI:CHEBI:58351, ChEBI:CHEBI:60049; EC=4.99.1.3; |
end case | |
PATHWAY | Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10. |
SUBUNIT | Homotetramer; dimer of dimers. |
SIMILARITY | Belongs to the CbiX family. CbiXS subfamily. |
Keywords
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Cobalamin biosynthesis | |
Lyase | |
case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
Methanogenesis | |
end case | |
case <FT:2> and <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
Metal-binding | |
Cobalt | |
Nickel | |
else | |
Metal-binding | |
Cobalt | |
end case |
Gene Ontology
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GO:0016852; Molecular function:sirohydrochlorin cobaltochelatase activity | |
GO:0019251; Biological process:anaerobic cobalamin biosynthetic process | |
case <FT:2> and <OCellular component:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | |
GO:0050897; Molecular function:cobalt ion binding | |
GO:0016151; Molecular function:nickel cation binding | |
else; https://www.ebi.ac.uk/QuickGO/term/else | |
GO:0050897; Molecular function:cobalt ion binding | |
end case |
Cross-references
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Pfam | PF01903; CbiX; 1; |
Features
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From: CBIX_ARCFU (O29537) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 69 | 74 | /ligand="substrate" | |||||||||
ACT_SITE | 10 | 10 | /note="Proton acceptor" | H | ||||||||
case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri> | ||||||||||||
BINDING | 10 | 10 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
BINDING | 10 | 10 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
H | ||||||||
BINDING | 74 | 74 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
BINDING | 74 | 74 | /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786" |
H | ||||||||
else | ||||||||||||
BINDING | 10 | 10 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
BINDING | 74 | 74 | /ligand="Co(2+)" /ligand_id="ChEBI:CHEBI:48828" |
H | ||||||||
end case | ||||||||||||
BINDING | 46 | 46 | /ligand="substrate" | [RKQE] |
Additional information
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Size range | 120-144 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |
Comments | HALSA has a protein with two tandem copies of a cbiX domain, resembling CbiK arrangement. |