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HAMAP rule MF_00965

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General rule information [?]

Accession MF_00965
Dates 12-JUN-2013 (Created)
19-NOV-2019 (Last updated, Version 5)
Name DEAD_helicase_DbpA
Scope(s) Bacteria
Template(s) P42305 (DBPA_BACSU); P21693 (DBPA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_00965 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DBPA
RecName: Full=ATP-dependent RNA helicase DbpA;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.6.4.13">3.6.4.13</a>;
Gene name Name=dbpA;

Comments [?]

FUNCTIONDEAD-box RNA helicase involved in the assembly of the 50S ribosomal subunit. Has an RNA-dependent ATPase activity, which is specific for 23S rRNA, and a 3' to 5' RNA helicase activity that uses the energy of ATP hydrolysis to destabilize and unwind short rRNA duplexes.
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
SUBCELLULAR LOCATIONCytoplasm.
DOMAINContains an N-terminal domain that binds non-specifically to RNA and a C-terminal domain that binds specifically and tightly to hairpin 92 of 23S rRNA.
SIMILARITYBelongs to the DEAD box helicase family. DbpA subfamily.

Keywords [?]

ATP-binding
Cytoplasm
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>
Hydrolase
Nucleotide-binding
Ribosome biogenesis
RNA-binding

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0034458; Molecular function:3'-5' RNA helicase activity
GO:0003723; Molecular function:RNA binding
GO:0000027; Biological process:ribosomal large subunit assembly
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS00039; DEAD_ATP_HELICASE; 1;
PROSITE PS51192; HELICASE_ATP_BIND_1; 1;
PROSITE PS51194; HELICASE_CTER; 1;
PROSITE PS51195; Q_MOTIF; 1;
Pfam PF03880; DbpA; 1;
Pfam PF00270; DEAD; 1;
Pfam PF00271; Helicase_C; 1;

Features [?]

From: DBPA_BACSU (P42305)
Key From To Description Tag Condition FTGroup
REGION 404 Cter /note="Involved in 23S rRNA binding"

Additional information [?]

Size range 430-529 amino acids
Related rules None
Fusion Nter: None Cter: None



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