HAMAP logo

HAMAP rule MF_01211

Send feedback

General rule information [?]

Accession MF_01211
Dates 17-JUN-2002 (Created)
19-NOV-2022 (Last updated, Version 46)
Name DHODB_Fe_S_bind
Scope(s) Bacteria
Archaea
Template(s) P56968 (PYRK_LACLM); P25983 (PYRK_BACSU); P0DH76 (PYRK_ENTFA); [ Recover all ]
Triggered by HAMAP; MF_01211 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PYRK
case <OC:Bacteria>
Protein name RecName: Full=Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit;
AltName: Full=Dihydroorotate oxidase B, electron transfer subunit;
end case
case <OC:Archaea>
Protein name RecName: Full=Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit;
AltName: Full=Dihydroorotate oxidase B, electron transfer subunit;
end case
Gene name Name=pyrK;

Comments [?]

FUNCTIONResponsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).
COFACTOR Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Note=Binds 1 [2Fe-2S] cluster per subunit.;
COFACTOR Name=FAD; Xref=ChEBI:CHEBI:57692; Note=Binds 1 FAD per subunit.;
PATHWAYPyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.
SUBUNITHeterotetramer of 2 PyrK and 2 PyrD type B subunits.
SIMILARITYBelongs to the PyrK family.

Keywords [?]


Gene Ontology [?]

GO:0009055; Molecular function:electron transfer activity
GO:0006221; Biological process:pyrimidine nucleotide biosynthetic process

Cross-references [?]

Pfam PF10418; DHODB_Fe-S_bind; 1;
Pfam PF00970; FAD_binding_6; 1;
Pfam PF00175; NAD_binding_1; 1;
PIRSF PIRSF006816; Cyc3_hyd_g; 1;
PRINTS PR00409; PHDIOXRDTASE; 1;
PROSITE PS00197; 2FE2S_FER_1; 0-1;
PROSITE PS51384; FAD_FR; 1;

Features [?]

From: PYRK_LACLM (P56968)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
BINDING 53 56 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"
R-P-x-S
BINDING 70 72 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"
[LIV]-x-[RQ]
BINDING 79 80 /ligand="FAD"
/ligand_id="ChEBI:CHEBI:57692"
G-T
end case
BINDING 226 226 /ligand="[2Fe-2S] cluster"
/ligand_id="ChEBI:CHEBI:190135"
C
BINDING 231 231 /ligand="[2Fe-2S] cluster"
/ligand_id="ChEBI:CHEBI:190135"
C
BINDING 234 234 /ligand="[2Fe-2S] cluster"
/ligand_id="ChEBI:CHEBI:190135"
C
BINDING 249 249 /ligand="[2Fe-2S] cluster"
/ligand_id="ChEBI:CHEBI:190135"
C

Additional information [?]

Size range 232-266 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Divergent THEMA and THEP1, annotated as "atypical" since they are quite divergent from the family profile, but can be thought to be PyrK proteins as the gene is adjacent to pyrD.



View rule in raw text format (no links)