AC MF_01211; DC Protein; auto TR HAMAP; MF_01211; -; 1; level=0 XX Names: DHODB_Fe_S_bind XX ID PYRK case DE RecName: Full=Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit; DE AltName: Full=Dihydroorotate oxidase B, electron transfer subunit; end case case DE RecName: Full=Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit; DE AltName: Full=Dihydroorotate oxidase B, electron transfer subunit; end case GN Name=pyrK; XX CC -!- FUNCTION: Responsible for channeling the electrons from the oxidation CC of dihydroorotate from the FMN redox center in the PyrD type B subunit CC to the ultimate electron acceptor NAD(+). CC -!- COFACTOR: CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; CC Note=Binds 1 [2Fe-2S] cluster per subunit.; CC -!- COFACTOR: CC Name=FAD; Xref=ChEBI:CHEBI:57692; CC Note=Binds 1 FAD per subunit.; CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; CC orotate from (S)-dihydroorotate (NAD(+) route): step 1/1. CC -!- SUBUNIT: Heterotetramer of 2 PyrK and 2 PyrD type B subunits. CC -!- SIMILARITY: Belongs to the PyrK family. XX DR Pfam; PF10418; DHODB_Fe-S_bind; 1; trigger=no DR Pfam; PF00970; FAD_binding_6; 1; trigger=no DR Pfam; PF00175; NAD_binding_1; 1; trigger=no DR PIRSF; PIRSF006816; Cyc3_hyd_g; 1; trigger=no DR PRINTS; PR00409; PHDIOXRDTASE; 1; trigger=no DR PROSITE; PS00197; 2FE2S_FER_1; 0-1; trigger=no DR PROSITE; PS51384; FAD_FR; 1; trigger=yes XX KW Pyrimidine biosynthesis KW Transport KW Electron transport KW Metal-binding KW Iron KW Iron-sulfur KW 2Fe-2S KW Flavoprotein KW FAD XX GO GO:0009055; F:electron transfer activity GO GO:0006221; P:pyrimidine nucleotide biosynthetic process XX FT From: PYRK_LACLM (P56968) case FT BINDING 53..56 FT /ligand="FAD" FT /ligand_id="ChEBI:CHEBI:57692" FT Condition: R-P-x-S FT BINDING 70..72 FT /ligand="FAD" FT /ligand_id="ChEBI:CHEBI:57692" FT Optional; Condition: [LIV]-x-[RQ] FT BINDING 79..80 FT /ligand="FAD" FT /ligand_id="ChEBI:CHEBI:57692" FT Condition: G-T end case FT BINDING 226 FT /ligand="[2Fe-2S] cluster" FT /ligand_id="ChEBI:CHEBI:190135" FT Condition: C FT BINDING 231 FT /ligand="[2Fe-2S] cluster" FT /ligand_id="ChEBI:CHEBI:190135" FT Condition: C FT BINDING 234 FT /ligand="[2Fe-2S] cluster" FT /ligand_id="ChEBI:CHEBI:190135" FT Condition: C FT BINDING 249 FT /ligand="[2Fe-2S] cluster" FT /ligand_id="ChEBI:CHEBI:190135" FT Condition: C XX Size: 232-266; Related: None; Template: P56968; P25983; P0DH76; Scope: Bacteria Archaea Fusion: Nter: None Cter: None Duplicate: None Plasmid: None Comments: Divergent THEMA and THEP1, annotated as "atypical" since they are quite divergent from the family profile, but can be thought to be PyrK proteins as the gene is adjacent to pyrD. XX # Revision 1.46 2022/11/19 //