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HAMAP rule MF_02043

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General rule information [?]

Accession MF_02043
Dates 20-JUL-2015 (Created)
26-JAN-2023 (Last updated, Version 6)
Name DusC_subfam
Scope(s) Bacteria
Pseudomonadota
Template(s) P33371 (DUSC_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_02043 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier DUSC
Protein name RecName: Full=tRNA-dihydrouridine(16) synthase;
                 EC=1.3.1.-;
AltName: Full=U16-specific dihydrouridine synthase;
                 Short=U16-specific Dus;
AltName: Full=tRNA-dihydrouridine synthase C;
Gene name Name=dusC;

Comments [?]

FUNCTIONCatalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs.
CATALYTIC ACTIVITY Reaction=5,6-dihydrouridine(16) in tRNA + NADP(+) = H(+) + NADPH + uridine(16) in tRNA; Xref=Rhea:RHEA:53376, Rhea:RHEA-COMP:13543, Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CATALYTIC ACTIVITY Reaction=5,6-dihydrouridine(16) in tRNA + NAD(+) = H(+) + NADH + uridine(16) in tRNA; Xref=Rhea:RHEA:53380, Rhea:RHEA-COMP:13543, Rhea:RHEA-COMP:13544, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
COFACTOR Name=FMN; Xref=ChEBI:CHEBI:58210;
SIMILARITYBelongs to the Dus family. DusC subfamily.

Keywords [?]


Gene Ontology [?]

GO:0010181; Molecular function:FMN binding
GO:0000049; Molecular function:tRNA binding
GO:0017150; Molecular function:tRNA dihydrouridine synthase activity
GO:0002943; Biological process:tRNA dihydrouridine synthesis

Cross-references [?]

PROSITE PS01136; UPF0034; 1;
Pfam PF01207; Dus; 1;
PIRSF PIRSF006621; Dus; 1;

Features [?]

From: DUSC_ECOLI (P33371)
Key From To Description Tag Condition FTGroup
BINDING 7 9 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
P-M-[EQ]
BINDING 200 202 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
N-G-[ED]
BINDING 224 225 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
[GC]-[RC]
ACT_SITE 98 98 /note="Proton donor" C
BINDING 68 68 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
Q
BINDING 139 139 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
[KR]
SITE 35 35 /note="Interacts with tRNA; defines subfamily-specific binding signature" R
SITE 95 95 /note="Interacts with tRNA" N
SITE 176 176 /note="Interacts with tRNA" Y
SITE 272 272 /note="Interacts with tRNA; defines subfamily-specific binding signature" R
SITE 274 274 /note="Interacts with tRNA; defines subfamily-specific binding signature" K
SITE 279 279 /note="Interacts with tRNA" Y
SITE 295 295 /note="Interacts with tRNA; defines subfamily-specific binding signature" [RK]

Additional information [?]

Size range 290-360 amino acids
Related rules None
Fusion Nter: None Cter: None



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