HAMAP: Caldicellulosiruptor kristjanssonii (strain ATCC 700853 / DSM 12137 / I77R1B) complete proteome

General information

Species:  Caldicellulosiruptor kristjanssonii (strain ATCC 700853 / DSM 12137 / I77R1B)
Species code: CALKI
Taxonomy: Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor (TaxID: 632335) [NEWT/ NCBI]
Description: Cellulolytic capability is widely distributed across the domain bacteria and extremely thermophilic cellulose-degrading microorganisms are of particular fundamental and biotechnological interest owing to the presence of highly thermostable enzymes. Caldicellulosiruptor kristjanssonii (strain ATCC 700853 / DSM 12137 / I77R1B) is a strictly anaerobic, cellulolytic, non-spore-forming, extremely thermophilic, Gram-positive bacterium isolated from a an enrichment culture grown at 78 degrees Celsius with Avicel as substrate and inoculated with a biomat sample from a slightly alkaline (pH 8.7) Icelandic hot spring. Cells are non-motile and rod-shaped cells with rounded ends, 2.8-9-4 pm long and 0.7-1.0 pm wide. Cells occur singly, in pairs and in short chains. Longer cells and chains of cells are observed under non-optimal conditions of temperature and pH. Colonies are 0.5-1.0 mm, flat, cream in color and had a fringed edge. C. kristjanssonii grows between 50-82 degrees Celsius with an optimum growth temperature around 78 degrees Celsius. At 70 degrees Celsius, growth occurs at pH 5.8-8.0 with an optimum pH at approximately 7.0. At 70 degrees Celsius, it is sensitive to chloramphenicol, neomycin, penicillin-S, streptomycin, tetracycline and vancomycin. During growth on Avicel, C. kristjanssonii produced acetate, hydrogen and carbon dioxide as major fermentation products together with small amounts of lactic acid and ethanol. The strain fermentes many substrates, including cellulose, xylan, starch and pectin, but does not grow with casein peptone, pyruvate, D-ribose or yeast extract and does not reduce thiosulfate to H2S. (Adapted from PMID: 21216991 and 10425755).
Properties: Presence of flagella: No
Human pathogen: No
Interaction: No
Number of membranes: 1
Statistics: Number of CALKI entries in UniProtKB: 2463 (0 in UniProtKB/Swiss-Prot + 2463 in UniProtKB/TrEMBL)

Genome(s) sequenced

Strain:    ATCC 700853 / DSM 12137 / I77R1B
Genome structure:
• Chromosome EMBL accession number CP002326 (circular; 2,786,473 bp) (download entry)
• Plasmid pCALKR01 EMBL accession number CP002327 (circular; 15,970 bp) (download entry)
Reference(s):
[1] Pubmed=21216991;
Blumer-Schuette S.E., Ozdemir I., Mistry D., Lucas S., Lapidus A., Cheng J.F., Goodwin L.A., Pitluck S., Land M.L., Hauser L.J., Woyke T., Mikhailova N., Pati A., Kyrpides N.C., Ivanova N., Detter J.C., Walston-Davenport K., Han S., Adams M.W., Kelly R.M. ;
"Complete genome sequences for the anaerobic, extremely thermophilic plant biomass-degrading bacteria Caldicellulosiruptor hydrothermalis, Caldicellulosiruptor kristjanssonii, Caldicellulosiruptor kronotskyensis, Caldicellulosiruptor owensenis, and Caldicellulosiruptor lactoaceticus.";
J. Bacteriol. 193:1483-1484(2011).
Web links:
Entrez Genome Project: http://www.ncbi.nlm.nih.gov/genomeprj/41727
GIB (DDBJ): http://gtps.ddbj.nig.ac.jp/single/index.php?spid=Ckri_177R1B


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