HAMAP: Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) complete proteome
General information

Species:  Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477)
Species code: PSEF5
Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas (TaxID: 220664) [NEWT/ NCBI]
Description: Pseudomonas fluorescens is a is a physiologically diverse species of opportunistic bacteria that colonizes soil, water and plant surface environments. Some isolates, such as SBW25 and Pf-5, are beneficial to plant health and nutrition. It produces a soluble, greenish fluorescent pigment, particularly under conditions of low iron availability. It is a obligate aerobe, except for some strains that can utilize NO3 as an electron acceptor in place of O2. It is motile by means of multiple polar flagella. It has simple nutritional requirements and grows well in mineral salts media supplemented with any of a large number of carbon sources. Pseudomonas fluorescens strains are being studied for use in applications that require release and survival of bacteria in the soil such as bioremediation of various organic compounds, and biocontrol of pathogens in agriculture. A number of strains suppress plant diseases by protecting the seeds and roots from fungal infection. Competitive exclusion of pathogens as the result of rapid colonization of the rhizosphere by Pseudomonas fluorescens may also be an important factor in disease control.

Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere of many plants and suppresses plant diseases caused by soilborne plant pathogens. It produces several antibiotics and secondary metabolites. Comparisons of the 3 available strains (as of June 2009, SBW25, Pf-5 and Pf0-1) indicate they share only 61% of their genes, most of which cluster near the origin of replication. In fact, the three P. fluorescens strains could be different species. 125 SBW25 genes have been shown to be upregulated in the presence of plants; 83 have orthologs in Pf0-1 and 73 have orthologs in Pf-5.
Properties: Presence of flagella: Yes
Human pathogen: No
Interaction: Plant commensal
Number of membranes: 2
Number of inteins:0
Statistics: Number of PSEF5 entries in the UniProt Knowledgebase: 5421 (546 in UniProtKB/Swiss-Prot + 4875 in UniProtKB/TrEMBL)


Genome(s) sequenced

Strain:    Pf-5 / ATCC BAA-477
Genome structure:
Chromosome EMBL accession number CP000076 (circular; 7,074,893 bp) (download entry)
Reference(s):
[1] PubMed=15980861; [ NCBI , EBI , Israel , Japan ]
Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A., Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J., Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J., Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C., Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III, Thomashow L.S., Loper J.E. ;
"Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.";
Nat. Biotechnol. 23:873-878(2005).
Web links:
CBS: http://www.cbs.dtu.dk/services/GenomeAtlas/show-genus.php?kingdom=Bacteria&GLgenus=Pseudomonas&GLspecies=fluorescens&GLstrain=Pf-5
Entrez Genome Project: http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genomeprj&Cmd=DetailsSearch&Term=txid220664%5Borgn%5D
GIB (DDBJ): http://gib.genes.nig.ac.jp/single/index.php?spid=Pful_PF5
JVCI CMR: http://cmr.jcvi.org/tigr-scripts/CMR/GenomePage.cgi?database=gpf
PEDANT: http://pedant.gsf.de/pedant3htmlview/pedant3view?Method=analysis&Db=p3_p327_Pse_fluor
 EBI Proteome Analysis page



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