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Annotation rule MF_00008
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General rule information [?]

Accession MF_00008
Dates 1-JUN-2001 (Created)
16-AUG-2016 (Last updated, Version 37)
Name Thymidy_synth_bact
Scope Bacteria
Archaea; Halobacteria
Templates P0CI79 (TYSY1_BACSU); P0A884 (TYSY_ECOLI); P00469 (TYSY_LACCA); P9WFR9 (TYSY_MYCTU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TYSY
Protein name
RecName: Full=Thymidylate synthase;
Short=TS;
Short=TSase;
EC=2.1.1.45;
Gene name
thyA

Comments [?]

Function Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
Catalytic activity 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway Pyrimidine metabolism; dTTP biosynthesis.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004799; Molecular function: thymidylate synthase activity.
GO:0006231; Biological process: dTMP biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS00091; THYMIDYLATE_SYNTHASE; 1;
Pfam PF00303; Thymidylat_synt; 1;
PRINTS PR00108; THYMDSNTHASE; 1;
TIGRFAMs TIGR03284; thym_sym; 1;

Features [?]

From: TYSY_ECOLI (P0A884)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     126     127       dUMP; shared with dimeric partner     R-R  
NP_BIND     166     169       dUMP     R-S-x-D  
NP_BIND     207     209       dUMP     H-x-Y  
ACT_SITE     146     146       Nucleophile     C  
BINDING (Optional)     21     21       dUMP     R  
BINDING (Optional)     51     51       5,10-methylenetetrahydrofolate     [HN]  
BINDING     169     169       5,10-methylenetetrahydrofolate     D  
BINDING     177     177       dUMP     N  
BINDING (Optional)     263     263       5,10-methylenetetrahydrofolate; via carbonyl oxygen     [AS]  

Additional information [?]

Size range 258-323 amino acids
Related rules MF_01686 (TYSY supersedes the current rule)
Fusion None
Comments Duplicates in Bacillus subtilis or Bacillus amyloliquefaciens are due to the presence of a phage-derived TS. See: PubMed=9648749. Tam N.H., Borriss R.; "Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families."; Mol. Gen. Genet. 258:427-430(1998). Archaeal thyA belong to a separate family (MF_01686)