HAMAP

Accession	MF_00011
Dates	1-JUN-2001 (Created) 9-DEC-2011 (Last updated, Version 41)

Data class

Protein

Predictors

HAMAP; MF_00011; [distribution of match scores in UniProtKB];[seed alignment for MF_00011]

Names

Adenylosucc_synth

Identifier

PURA

Protein name

RecName: Full=Adenylosuccinate synthetase; Short=AMPSase; Short=AdSS; EC=6.3.4.4; AltName: Full=IMP--aspartate ligase;

Gene name

purA

FUNCTION: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP (By similarity).

CATALYTIC ACTIVITY: GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the adenylosuccinate synthetase family.

Pfam	PF00709; Adenylsucc_synt; 1;
TIGRFAMs	TIGR00184; PurA; 1;
PROSITE	PS01266; ADENYLOSUCCIN_SYN_1; 1; PS00513; ADENYLOSUCCIN_SYN_2; 1;

Cytoplasm, Purine biosynthesis, Ligase, GTP-binding, Nucleotide-binding, Magnesium, Metal-binding.

GO:0004019; Molecular function: adenylosuccinate synthase activity.
GO:0005525; Molecular function: GTP binding.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006164; Biological process: purine nucleotide biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: PURA_ECOLI (P0A7D4)
Key		From		To		Description		Condition		FTGroup
NP_BIND		13		19		GTP (By similarity)		G-D-E-[GA]-K-[GA]-x
NP_BIND		41		43		GTP (By similarity)		G-H-x
NP_BIND		332		334		GTP (By similarity)		x-x-D
NP_BIND		415		417		GTP (By similarity)		x-x-[GS]
REGION		14		17		IMP binding (By similarity)		D-E-[GA]-K
REGION		39		42		IMP binding (By similarity)		N-[AS]-G-H
REGION		300		306		Substrate binding (By similarity)		x-x-[ST]-x-[KR]-x-R
ACT_SITE		14		14		Proton acceptor (By similarity)		D
ACT_SITE		42		42		Proton donor (By similarity)		H
METAL		14		14		Magnesium (By similarity)		D
METAL		41		41		Magnesium; via carbonyl oxygen (By similarity)		G
BINDING		130		130		IMP (By similarity)		T
BINDING		144		144		IMP; shared with dimeric partner (By similarity)		[KR]
BINDING		225		225		IMP (By similarity)		[QN]
BINDING		240		240		IMP (By similarity)		T
BINDING		304		304		IMP (By similarity)		[KR]
BINDING		306		306		GTP (By similarity)		R

Size range:	336-459 amino acids
Related UniRules:	MF_04126 (PURA1 (supersedes the current rule)); MF_04127 (PURA2 (supersedes the current rule)); MF_04125 (PURA)
Template:	P0A7D4 (PURA_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in CHRVO, PHOLL, METAC
Plasmid encoded:	None