HAMAP rule MF_00015
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00015 |
| Accession | MF_00015 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 45) |
| Name | LexA |
| Scope(s) |
Bacteria |
| Template(s) | P31080 (LEXA_BACSU); P0A7C2 (LEXA_ECOLI); Q9ZFA4 (LEXA_CERS4); [ Recover all ] |
| Triggered by |
HAMAP; MF_00015 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | LEXA |
| Protein name | RecName: Full=LexA repressor; EC=3.4.21.88; |
| Gene name | Name=lexA; |
Comments
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| case <OC:Enterobacterales> | |
| FUNCTION | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single- stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. |
| else | |
| FUNCTION | Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=Hydrolysis of Ala-|-Gly bond in repressor LexA.; EC=3.4.21.88; |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the peptidase S24 family. |
Keywords
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| Transcription |
| Transcription regulation |
| Repressor |
| DNA-binding |
| DNA damage |
| Autocatalytic cleavage |
| Hydrolase |
| DNA replication |
| DNA repair |
| SOS response |
Gene Ontology
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| GO:0003677; Molecular function:DNA binding |
| GO:0004252; Molecular function:serine-type endopeptidase activity |
| GO:0006281; Biological process:DNA repair |
| GO:0006260; Biological process:DNA replication |
| GO:0045892; Biological process:negative regulation of DNA-templated transcription |
| GO:0006974; Biological process:DNA damage response |
| GO:0009432; Biological process:SOS response |
Cross-references
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| Pfam | PF01726; LexA_DNA_bind; 1; |
| Pfam | PF00717; Peptidase_S24; 1; |
| PRINTS | PR00726; LEXASERPTASE; 1; |
| NCBIfam | TIGR00498; LexA; 1; |
Features
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| From: LEXA_ECOLI (P0A7C2) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DNA_BIND | 28 | 48 | /note="H-T-H motif" | |||||||||
| ACT_SITE | 119 | 119 | /note="For autocatalytic cleavage activity" | S | ||||||||
| ACT_SITE | 156 | 156 | /note="For autocatalytic cleavage activity" | K | ||||||||
| SITE | 84 | 85 | /note="Cleavage; by autolysis" | A-G | ||||||||
Additional information
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| Size range | 192-275 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |