HAMAP annotation rule MF_00021
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Accession MF_00021
Dates 1-JUN-2001 (Created)
6-JAN-2012 (Last updated, Version 34)
Name ThiI
Scope Bacteria
Archaea
Triggered by



Identifier
THII
case <Feature:PS50206>
Protein name
RecName: Full=tRNA sulfurtransferase;
EC=2.8.1.4;
AltName: Full=Sulfur carrier protein ThiS sulfurtransferase;
AltName: Full=Thiamine biosynthesis protein ThiI;
AltName: Full=tRNA 4-thiouridine synthase;
end case
case not <Feature:PS50206>
Protein name
RecName: Full=Probable tRNA sulfurtransferase;
EC=2.8.1.4;
AltName: Full=Sulfur carrier protein ThiS sulfurtransferase;
AltName: Full=Thiamine biosynthesis protein ThiI;
AltName: Full=tRNA 4-thiouridine synthase;
end case
Gene name
thiI


FUNCTION Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS (By similarity).
CATALYTIC ACTIVITY L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.
PATHWAY Cofactor biosynthesis; thiamine diphosphate biosynthesis.
SUBCELLULAR LOCATION Cytoplasm (By similarity).
SIMILARITY Belongs to the ThiI family.
case <Feature:PS50206>
SIMILARITY Contains 1 rhodanese domain.
end case
case <Feature:PS51165>
SIMILARITY Contains 1 THUMP domain.
end case


case <Feature:PS51165>
RNA-binding
tRNA-binding
end case
ATP-binding
Cytoplasm
case <FT:7>
Disulfide bond
Redox-active center
end case
Nucleotide-binding
Thiamine biosynthesis
Transferase


case <Feature:PS51165>
GO:0000049; Molecular function: tRNA binding.
end case
GO:0005524; Molecular function: ATP binding.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.


PROSITE PS50206; RHODANESE_3; 0-1; trigger=PRU00173;
PS51165; THUMP; 1; trigger=PRU00529;
Pfam PF02568; ThiI; 1;
PF02926; THUMP; 1;
PF00581; Rhodanese; 1;
TIGRFAMs TIGR00342; TIGR00342; 1;


From: THII_BACAN (Q81KU0)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     183     184       ATP (By similarity)     [LM]-x  
NP_BIND (Optional)     208     209       ATP (By similarity)     [HYNTS]-[FY]  
BINDING     265     265       ATP (By similarity)     [RK]  
BINDING     287     287       ATP; via amide nitrogen (By similarity)     G  
BINDING     296     296       ATP (By similarity)     Q  
case <Feature:PS50206>
From: THII_ECOLI (P77718)
ACT_SITE     456     456       Cysteine persulfide intermediate (By similarity)     C  
DISULFID     344     456       Redox-active (By similarity)     C-x*-C  
end case


Size range: 368-486 amino acids
Related Rules: None
Templates: P77718 (THII_ECOLI); P55913 (THII_SALTY); Q81KU0 (THII_BACAN): [Recover all]
Fusion: None
Comments: Some proteins of this family do not possess a rhodanese domain, so there must be an external rhodanese-like partner involved. It is not known, and the reaction has not been characterized in this case.


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