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HAMAP annotation rule: MF_00021
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General rule information

Accession MF_00021
Dates 1-JUN-2001 (Created)
22-JUN-2011 (Last updated, Version 33)
Data class Protein
Predictors HAMAP; MF_00021; [distribution of match scores in UniProtKB];[seed alignment for MF_00021]
Names ThiI


Propagated annotation

Identifier, protein and gene names
Identifier THII

case <Feature:PS50206>
Protein name
RecName: Full=tRNA sulfurtransferase;
EC=2.8.1.4;
AltName: Full=Sulfur carrier protein ThiS sulfurtransferase;
AltName: Full=Thiamine biosynthesis protein thiI;
AltName: Full=tRNA 4-thiouridine synthase;
end case


case not <Feature:PS50206>
Protein name
RecName: Full=Probable tRNA sulfurtransferase;
EC=2.8.1.4;
AltName: Full=Sulfur carrier protein ThiS sulfurtransferase;
AltName: Full=Thiamine biosynthesis protein thiI;
AltName: Full=tRNA 4-thiouridine synthase;
end case

Gene name thiI
Comments
FUNCTION: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by iscS (By similarity).
CATALYTIC ACTIVITY: L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.
CATALYTIC ACTIVITY: [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.
PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the thiI family.

case <Feature:PS50206>
SIMILARITY: Contains 1 rhodanese domain.
end case


case <Feature:PS51165>
SIMILARITY: Contains 1 THUMP domain.
end case

Cross-references
PROSITE PS50206; RHODANESE_3; 0-1; trigger=PRU00173;
PS51165; THUMP; 1; trigger=PRU00529;
Pfam PF02568; ThiI; 1;
PF02926; THUMP; 1;
PF00581; Rhodanese; 1;
TIGRFAMs TIGR00342; TIGR00342; 1;
Keywords

case <Feature:PS51165>
RNA-binding, tRNA-binding.
end case

ATP-binding, Cytoplasm.

case <FT:7>
Disulfide bond, Redox-active center.
end case

Nucleotide-binding, Thiamine biosynthesis, Transferase.
Gene Ontology

case <Feature:PS51165>
GO:0000049; Molecular function: tRNA binding.
end case

GO:0005524; Molecular function: ATP binding.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0009228; Biological process: thiamine biosynthetic process.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
Features
From: THII_BACAN (Q81KU0)
Key     From     To       Description   Condition   FTGroup
NP_BIND     183     184       ATP (By similarity)   [LM]-x  
NP_BIND (Optional)     208     209       ATP (By similarity)   [HYNTS]-[FY]  
BINDING     265     265       ATP (By similarity)   [RK]  
BINDING     287     287       ATP; via amide nitrogen (By similarity)   G  
BINDING     296     296       ATP (By similarity)   Q  
case <Feature:PS50206>
From: THII_ECOLI (P77718)
Key     From     To       Description   Condition   FTGroup
ACT_SITE     456     456       Cysteine persulfide intermediate (By similarity)   C  
DISULFID     344     456       Redox-active (By similarity)   C-x*-C  
end case



Additional information

Size range: 368-486 amino acids
Related UniRules: None
Template: P77718 (THII_ECOLI); P55913 (THII_SALTY); Q81KU0 (THII_BACAN): [Recover all]
Scope: Bacteria
Archaea
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None
Comments: Some proteins of this family do not possess a rhodanese domain, so there must be an external rhodanese-like partner involved. It is not known, and the reaction has not been characterized in this case.

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UniProtKB rule member sequences