HAMAP rule MF_00022
General rule information
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| Accession | MF_00022 |
| Dates | 1-JUN-2001 (Created)
3-SEP-2024 (Last updated, Version 51) |
| Name | Glu_tRNA_synth_type1 |
| Scope(s) |
Bacteria |
| Template(s) | P04805 (SYE_ECOLI); O25360 (SYE2_HELPY); P27000 (SYE_THET8); Q8DLI5 (SYE_THEVB); [ Recover all ] |
| Triggered by |
HAMAP; MF_00022 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYE |
| Protein name | RecName: Full=Glutamate--tRNA ligase; EC=6.1.1.17; AltName: Full=Glutamyl-tRNA synthetase; Short=GluRS; |
| Gene name | Name=gltX; |
Comments
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| FUNCTION | Catalyzes the attachment of glutamate to tRNA(Glu) in a two- step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu). |
| CATALYTIC ACTIVITY | Reaction=tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate; Xref=Rhea:RHEA:23540, Rhea:RHEA-COMP:9663, Rhea:RHEA-COMP:9680, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78520, ChEBI:CHEBI:456215; EC=6.1.1.17; |
| case <FTGroup:1> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| end case | |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily. |
Keywords
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| Aminoacyl-tRNA synthetase | |
| ATP-binding | |
| Cytoplasm | |
| Ligase | |
| Nucleotide-binding | |
| Protein biosynthesis | |
| case <FTGroup:1> | |
| Metal-binding | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding | |
| GO:0004818; Molecular function:glutamate-tRNA ligase activity | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0006424; Biological process:glutamyl-tRNA aminoacylation | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| Pfam | PF00749; tRNA-synt_1c; 1; |
| PRINTS | PR00987; TRNASYNTHGLU; 1; |
| NCBIfam | TIGR00464; GltX_bact; 1; |
| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
Features
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| From: SYE_ECOLI (P04805) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 9 | 19 | /note="'HIGH' region" | P-[SN]-P-x(4)-[HR]-[LIVMAT]-[GE]-x | ||||||||
| MOTIF | 237 | 241 | /note="'KMSKS' region" | x-[LIMSF]-S-K-[KR] | ||||||||
| BINDING | 98 | 98 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 100 | 100 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 125 | 125 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
[CH] | 1 | |||||||
| BINDING | 127 | 127 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
[HED] | 1 | |||||||
| BINDING | 240 | 240 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
Additional information
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| Size range | 431-546 amino acids |
| Related rules |
MF_01428 |
| Fusion | Nter: None Cter: <Unknown> |
| Comments | This signature is specific for bacterial and eukaryotic mitochondrial glutamate--tRNA ligases. See MF_02076 for archaeal and eukaryotic cytoplasmic glutamate--tRNA ligases. |