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HAMAP rule MF_00023

General rule information [?]

Accession	MF_00023
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 26)
Name	SmpB
Scope(s)	Bacteria
Template(s)	P0A832 (SSRP_ECOLI); Q8RR57 (SSRP_THET8); O66640 (SSRP_AQUAE); O32230 (SSRP_BACSU); [ Recover all ]
Triggered by	HAMAP; MF_00023 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	SSRP
Protein name	RecName: Full=SsrA-binding protein; AltName: Full=Small protein B;
Gene name	Name=smpB;

Comments [?]

FUNCTION	Required for rescue of stalled ribosomes mediated by trans- translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans- translation.
SUBCELLULAR LOCATION	Cytoplasm. Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes.
SIMILARITY	Belongs to the SmpB family.

Keywords [?]

Gene Ontology [?]

GO:0003723; Molecular function:RNA binding

GO:0005737; Cellular component:cytoplasm

GO:0070929; Biological process:trans-translation

Cross-references [?]

Features [?]

Additional information [?]