HAMAP rule MF_00031
General rule information
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| Accession | MF_00031 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 29) |
| Name | DNA_HJ_migration_RuvA |
| Scope(s) |
Bacteria |
| Template(s) | P0A809 (RUVA_ECOLI); P40832 (RUVA_MYCLE); Q9F1Q3 (RUVA_THET8); P9WGW3 (RUVA_MYCTU); P66746 (RUVA_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_00031 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RUVA |
| Protein name | RecName: Full=Holliday junction branch migration complex subunit RuvA; |
| Gene name | Name=ruvA; |
Comments
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| FUNCTION | The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair, while the RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR). RuvA specifically binds to HJ cruciform DNA, conferring on it an open structure. The RuvB hexamer acts as an ATP-dependent pump, pulling dsDNA into and through the RuvAB complex. HJ branch migration allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA. |
| SUBUNIT | Homotetramer. Forms an RuvA(8)-RuvB(12)-Holliday junction (HJ) complex. HJ DNA is sandwiched between 2 RuvA tetramers; dsDNA enters through RuvA and exits via RuvB. An RuvB hexamer assembles on each DNA strand where it exits the tetramer. Each RuvB hexamer is contacted by two RuvA subunits (via domain III) on 2 adjacent RuvB subunits; this complex drives branch migration. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| DOMAIN | Has three domains with a flexible linker between the domains II and III and assumes an 'L' shape. Domain III is highly mobile and contacts RuvB. |
| SIMILARITY | Belongs to the RuvA family. |
Keywords
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| Cytoplasm | |
| DNA damage | |
| DNA repair | |
| case <OC:Escherichia> | |
| SOS response | |
| end case | |
| DNA-binding | |
| DNA recombination | |
Gene Ontology
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| GO:0005737; Cellular component:cytoplasm | |
| GO:0000400; Molecular function:four-way junction DNA binding | |
| GO:0048476; Cellular component:Holliday junction resolvase complex | |
| GO:0006310; Biological process:DNA recombination | |
| GO:0006281; Biological process:DNA repair | |
| case <OCellular component:Escherichia> | |
| GO:0009432; Biological process:SOS response | |
| end case | |
Cross-references
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Features
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| From: RUVA_ECOLI (P0A809) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 1 | 64 | /note="Domain I" | |||||||||
| REGION | 65 | 142 | /note="Domain II" | |||||||||
| REGION | 143 | 155 | /note="Flexible linker" | |||||||||
| REGION | 156 | Cter | /note="Domain III" | |||||||||
Additional information
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| Size range | 150-250 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |