HAMAP annotation rule MF_00034
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Accession MF_00034
Dates 1-JUN-2001 (Created)
18-MAR-2013 (Last updated, Version 25)
Name RuvC
Scope Bacteria
Triggered by



Identifier
RUVC
Protein name
RecName: Full=Crossover junction endodeoxyribonuclease RuvC;
EC=3.1.22.4;
AltName: Full=Holliday junction nuclease RuvC;
AltName: Full=Holliday junction resolvase RuvC;
Gene name
ruvC


FUNCTION Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group (By similarity).
CATALYTIC ACTIVITY Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
case <FTGroup:1>
COFACTOR Binds 1 magnesium ion per subunit (By similarity).
end case
SIMILARITY Belongs to the RuvC family.


Hydrolase
Nuclease
DNA damage
DNA repair
DNA recombination
case <FTGroup:1>
Magnesium
Metal-binding
end case


GO:0008821; Molecular function: crossover junction endodeoxyribonuclease activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0006310; Biological process: DNA recombination.
GO:0006281; Biological process: DNA repair.


Pfam PF02075; RuvC; 1;
TIGRFAMs TIGR00228; ruvC; 1;
PROSITE PS01321; RUVC; 1;


From: RUVC_ECOLI (P0A814)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     8     8       Magnesium (By similarity)     D   1
METAL     67     67       Magnesium (By similarity)     E   1
METAL     139     139       Magnesium (By similarity)     [DH]   1
METAL     142     142       Magnesium (By similarity)     D   1


Size range: 100-300 amino acids
Related Rules: None
Template: P0A814 (RUVC_ECOLI)
Fusion: None


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