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HAMAP annotation rule: MF_00041
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General rule information

Accession MF_00041
Dates 1-JUN-2001 (Created)
21-NOV-2011 (Last updated, Version 30)
Data class Protein
Predictors HAMAP; MF_00041; [distribution of match scores in UniProtKB];[seed alignment for MF_00041]
Names Cys_tRNA_synth


Propagated annotation

Identifier, protein and gene names
Identifier SYC
Protein name
RecName: Full=Cysteine--tRNA ligase;
EC=6.1.1.16;
AltName: Full=Cysteinyl-tRNA synthetase;
Short=CysRS;
Gene name cysS
Comments
CATALYTIC ACTIVITY: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).

case <FTGroup:1> or <FTGroup:2>
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
end case


case <OC:Bacteria>
SUBUNIT: Monomer (By similarity).
end case

SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
Cross-references
Pfam PF01406; tRNA-synt_1e; 1;
PRINTS PR00983; TRNASYNTHCYS; 1;
TIGRFAMs TIGR00435; CysS; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
Keywords
Cytoplasm, Aminoacyl-tRNA synthetase, Protein biosynthesis, Ligase, ATP-binding, Nucleotide-binding.

case <FTGroup:1> or <FTGroup:2>
Metal-binding, Zinc.
end case


case <FT:8>
Phosphoprotein.
end case

Gene Ontology
GO:0005524; Molecular function: ATP binding.
GO:0004817; Molecular function: cysteine-tRNA ligase activity.
GO:0006423; Biological process: cysteinyl-tRNA aminoacylation.

case <FTGroup:1> or <FTGroup:2>
GO:0008270; Molecular function: zinc ion binding.
end case

GO:0005737; Cellular component: cytoplasm.
Features
From: SYC_ECOLI (P21888)
Key     From     To       Description   Condition   FTGroup
MOTIF     30     40       "HIGH" region   x-T-x(5)-H-[LIVFA]-G-[HN]  
MOTIF     266     270       "KMSKS" region   [KR]-M-[GSA]-[KS]-S  
 
METAL     28     28       Zinc (By similarity)   C   1
METAL     209     209       Zinc (By similarity)   C   1
METAL     234     234       Zinc (By similarity)   H   1
METAL     238     238       Zinc (By similarity)   E   1
 
BINDING     269     269       ATP (By similarity)   [KS]  
case <OC:Bacillaceae>
From: SYC_BACSU (Q06752)
Key     From     To       Description   Condition   FTGroup
MOD_RES     270     270       Phosphoserine (By similarity)   S  
end case



Additional information

Size range: 404-546 amino acids
Related UniRules: None
Template: P21888 (SYC_ECOLI)
Scope: Bacteria
Archaea
Fusion: Nter: None; Cter: None
Duplicate: in BURS3, PHOPR, TROW8, TROWT
Plasmid encoded: None
Comments: Atypical sequence in STRCO that contains a weird additional N-terminal domain constituted of multiple repeats; not taken into account in size range For archeal missing cysS see: MEDLINE=99412295; PubMed=10482537; Hamann C.S., Sowers K.R., Lipman R.S., Hou Y.M.; "An archaeal aminoacyl-tRNA synthetase missing from genomic analysis."; J. Bacteriol. 181:5880-5884(1999). MEDLINE=20086427; PubMed=10622715; Li T., Graham D.E., Stathopoulos C., Haney P.J., Kim H.S., Vothknecht U., Kitabatake M., Hong K.W., Eggertsson G., Curnow A.W., Lin W., Celic I., Whitman W., Soll D.; "Cysteinyl-tRNA formation: the last puzzle of aminoacyl-tRNA synthesis."; FEBS Lett. 462:302-306(1999).

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UniProtKB rule member sequences