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HAMAP rule MF_00049

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General rule information [?]

Accession MF_00049
Dates 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 36)
Name Leu_tRNA_synth
Scope(s) Bacteria
Archaea
Template(s) P07813 (SYL_ECOLI); [ Recover all ]
Triggered by
case c? <OC:Bacteria>
HAMAP; MF_00049_B (Get profile general information and statistics)
end case
case c? <OC:Archaea>
HAMAP; MF_00049_A (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYL
Protein name RecName: Full=Leucine--tRNA ligase;
                 EC=6.1.1.4;
AltName: Full=Leucyl-tRNA synthetase;
                 Short=LeuRS;
Gene name Name=leuS;

Comments [?]

CATALYTIC ACTIVITY Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl- tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA- COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427, ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the class-I aminoacyl-tRNA synthetase family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004823; Molecular function:leucine-tRNA ligase activity
GO:0006429; Biological process:leucyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR00985; TRNASYNTHLEU; 1;
NCBIfam TIGR00395; LeuS_arch; 1;
NCBIfam TIGR00396; LeuS_bact; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;

Features [?]

From: SYL_ECOLI (P07813)
Key From To Description Tag Condition FTGroup
case <OC:Bacteria>
MOTIF 42 52 /note="'HIGH' region" [SP]-[YF]-[PLVT]-[SN]-[GAS]-x(1,3)-H-[LIVMA]-G-[HS]
MOTIF 619 623 /note="'KMSKS' region" [KRTA]-[MLI]-[SGA]-[KS]-[SA]
BINDING 622 622 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
K
end case
From: SYL_METJA (Q58050)
Key From To Description Tag Condition FTGroup
case <OC:Archaea>
MOTIF 41 51 /note="'HIGH' region" P-Y-x-[SN]-[GAS]-x(2)-H-[LIVMA]-G-[HS]
MOTIF 633 637 /note="'KMSKS' region"
BINDING 636 636 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
K
end case

Additional information [?]

Size range 910-977 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments The AQUAE leuS is cut into two subunits. It is not shown in the alignment. There is a shorter second copy in SULTO, which is not shown in the alignment, nor are HALSA or NANEQ as they are quite divergent.



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