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HAMAP annotation rule: MF_00049
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General rule information

Accession MF_00049
Dates 1-JUN-2001 (Created)
21-NOV-2011 (Last updated, Version 28)
Data class Protein

case <OC:Bacteria>
Predictors HAMAP; MF_00049_B; [distribution of match scores in UniProtKB];[seed alignment for MF_00049_B]
end case


case <OC:Archaea>
Predictors HAMAP; MF_00049_A; [distribution of match scores in UniProtKB];[seed alignment for MF_00049_A]
end case

Names Leu_tRNA_synth


Propagated annotation

Identifier, protein and gene names
Identifier SYL
Protein name
RecName: Full=Leucine--tRNA ligase;
EC=6.1.1.4;
AltName: Full=Leucyl-tRNA synthetase;
Short=LeuRS;
Gene name leuS
Comments
CATALYTIC ACTIVITY: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
Cross-references
Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR00985; TRNASYNTHLEU; 1;
TIGRFAMs TIGR00395; LeuS_arch; 1;
TIGR00396; LeuS_bact; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
Keywords
Cytoplasm, Aminoacyl-tRNA synthetase, Protein biosynthesis, Ligase, ATP-binding, Nucleotide-binding.
Gene Ontology
GO:0005524; Molecular function: ATP binding.
GO:0004823; Molecular function: leucine-tRNA ligase activity.
GO:0006429; Biological process: leucyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Features

case <OC:Bacteria>
From: SYL_ECOLI (P07813)
Key     From     To       Description   Condition   FTGroup
MOTIF     42     52       "HIGH" region   [SP]-[YF]-[PLVT]-[SN]-[GAS]-x(1,3)-H-[LIVMA]-G-[HS]  
MOTIF     619     623       "KMSKS" region   [KRTA]-[MLI]-[SGA]-[KS]-[SA]  
BINDING     622     622       ATP (By similarity)   K  
end case

case <OC:Archaea>
From: SYL_METJA (Q58050)
Key     From     To       Description   Condition   FTGroup
MOTIF     41     51       "HIGH" region   P-Y-x-[SN]-[GAS]-x(2)-H-[LIVMA]-G-[HS]  
MOTIF     633     637       "KMSKS" region      
BINDING     636     636       ATP (By similarity)   K  
end case



Additional information


case <OC:Bacteria>
Size range: 792-990 amino acids
end case


case <OC:Archaea>
Size range: 910-977 amino acids
end case

Related UniRules: None
Template: P07813 (SYL_ECOLI)
Scope: Bacteria
Archaea
Fusion: Nter: None; Cter: None
Duplicate: in RHIL3, METS5, SULSO, SULTO
Plasmid encoded: None
Comments: The AQUAE leuS is cut into two subunits. It is not shown in the alignment. There is a shorter second copy in SULTO, which is not shown in the alignment, nor are HALSA or NANEQ as they are quite divergent.

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UniProtKB rule member sequences