HAMAP

Accession	MF_00051
Dates	1-JUN-2001 (Created) 5-DEC-2011 (Last updated, Version 41)

Data class

Protein

Predictors

HAMAP; MF_00051; [distribution of match scores in UniProtKB];[seed alignment for MF_00051]

Names

SHMT

Identifier

GLYA

Protein name

RecName: Full=Pyridoxal-phosphate-dependent serine hydroxymethyltransferase; Short=SHMT; Short=Serine methylase; EC=2.1.2.1;

Gene name

glyA

FUNCTION: Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier (By similarity).

CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.

COFACTOR: Pyridoxal phosphate (By similarity).

PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.

PATHWAY: Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the SHMT family.

Pfam	PF00464; SHMT; 1;
PROSITE	PS00096; SHMT; 1;
PIRSF	PIRSF000412; SHMT; 1;

Acetylation.

Amino-acid biosynthesis, Cytoplasm, One-carbon metabolism, Pyridoxal phosphate, Transferase.

GO:0004372; Molecular function: glycine hydroxymethyltransferase activity.
GO:0006730; Biological process: one-carbon metabolic process.
GO:0009069; Biological process: serine family amino acid metabolic process.
GO:0005737; Cellular component: cytoplasm.

From: GLYA_ECOLI (P0A825)
Key		From		To		Description		Condition		FTGroup
REGION		125		127		Substrate binding (By similarity)		G-X-[IL]

Key		From		To		Description		Condition		FTGroup
REGION		355		357		Substrate binding (By similarity)		S-X-F

Key		From		To		Description		Condition		FTGroup
BINDING (Optional)		35		35		Pyridoxal phosphate (By similarity)		S
BINDING		55		55		Pyridoxal phosphate (By similarity)		Y
BINDING		57		57		Substrate (By similarity)		E
BINDING		64		64		Substrate binding (By similarity)		[FY]
BINDING		65		65		Pyridoxal phosphate (By similarity)		Y
BINDING (Optional)		99		99		Pyridoxal phosphate (By similarity)		S
BINDING		121		121		Substrate (By similarity)		L
BINDING		175		175		Pyridoxal phosphate (By similarity)		[ST]
BINDING		203		203		Pyridoxal phosphate (By similarity)		H
BINDING (Optional)		228		228		Pyridoxal phosphate (By similarity)		H
BINDING (Optional)		235		235		Pyridoxal phosphate (By similarity)		R
BINDING (Optional)		263		263		Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen (By similarity)		G
BINDING		363		363		Pyridoxal phosphate (By similarity)		R
MOD_RES		229		229		N6-(pyridoxal phosphate)lysine (By similarity)		K

Key		From		To		Description		Condition		FTGroup
MOD_RES		54		54		N6-acetyllysine (By similarity)		K
MOD_RES		250		250		N6-acetyllysine (By similarity)		K
MOD_RES		285		285		N6-acetyllysine (By similarity)		K
MOD_RES		354		354		N6-acetyllysine (By similarity)		K
MOD_RES		375		375		N6-acetyllysine (By similarity)		K

Size range:	406-574 amino acids
Related UniRules:	None
Template:	P0A825 (GLYA_ECOLI)
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in AGRT5, BORBR, BORPA, BURMA, BURP1, BURPS, BURS3, BURTA, COLP3, ERWCT, HAHCH, MYCBO, MYCTU, PHOPR, PSE14, PSEAE, PSEF5, PSEPF, PSEPK, PSESM, PSEU2, CUPPJ, RALSO, RHILO, RHIME, RHOPA, RHORT, SILPO, SULDN, VIBCH, VIBPA, VIBVU, VIBVY
Plasmid encoded:	None
Comments:	Possible wrong start in ARCFU. Possible C-terminal problem in TREPA.