HAMAP rule MF_00054
General rule information
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| Accession | MF_00054 |
| Dates | 1-JUN-2001 (Created)
28-NOV-2023 (Last updated, Version 43) |
| Name | EF_G_EF_2 |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0A6M8 (EFG_ECOLI); P13551 (EFG_THETH); P30925 (EF2_SACS2); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_00054_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_00054_A (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Bacteria> | |
| Identifier | EFG |
| Protein name | RecName: Full=Elongation factor G; Short=EF-G; |
| end case | |
| case <OC:Archaea> | |
| Identifier | EF2 |
| Protein name | RecName: Full=Elongation factor 2; Short=EF-2; |
| end case | |
| Gene name | Name=fusA; |
Comments
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| FUNCTION | Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily. |
Keywords
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Gene Ontology
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| GO:0005525; Molecular function:GTP binding |
| GO:0003746; Molecular function:translation elongation factor activity |
| GO:0006414; Biological process:translational elongation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF00679; EFG_C; 1; |
| Pfam | PF00009; GTP_EFTU; 1; |
| Pfam | PF03144; GTP_EFTU_D2; 1; |
| PRINTS | PR00315; ELONGATNFCT; 1; |
| NCBIfam | TIGR00231; Small_GTP; 1; |
| NCBIfam | TIGR00484; EF-G; 1; |
| NCBIfam | TIGR00490; AEF-2; 1; |
| PROSITE | PS00301; G_TR_1; 1; |
| PROSITE | PS51722; G_TR_2; 1; |
Features
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| From: EFG_ECOLI (P0A6M8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> | ||||||||||||
| BINDING | 17 | 24 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[AS]-H-[IV]-D-[AS]-G-K-T | ||||||||
| BINDING | 88 | 92 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-T-P-G-H | ||||||||
| BINDING | 142 | 145 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
N-K-x-D | ||||||||
| end case | ||||||||||||
| From: EF2_SACS2 (P30925) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| BINDING | 27 | 34 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
A-H-[IV]-[DH]-H-G-K-T | ||||||||
| BINDING | 93 | 97 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-T-P-G-H | ||||||||
| BINDING | 147 | 150 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
N-K-[IV]-D | ||||||||
| MOD_RES | 603 | 603 | /note="Diphthamide" | H | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 726-743 amino acids |
| Related rules |
MF_03063 |
| Fusion | Nter: None Cter: None |
| Comments | EFG2_BORBU could be made slightly longer in the N-terminal (MVVVGDL) by frameshifting just before the current initiator Met |