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HAMAP rule MF_00057

General rule information [?]

Accession	MF_00057
Dates	1-JUN-2001 (Created) 1-JUN-2023 (Last updated, Version 33)
Name	KdsB
Scope(s)	Bacteria
Template(s)	P04951 (KDSB_ECOLI); P42216 (KPSU5_ECOLX); Q8EEA9 (KDSB_SHEON); [ Recover all ]
Triggered by	HAMAP; MF_00057 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	KDSB
case <OC:Shewanella>
Protein name	RecName: Full=8-amino-3,8-dideoxy-manno-octulosonate cytidylyltransferase; EC=2.7.7.90; AltName: Full=CMP-8-amino-3,8-dideoxy-manno-octulosonate synthase;
else
Protein name	RecName: Full=3-deoxy-manno-octulosonate cytidylyltransferase; EC=2.7.7.38; AltName: Full=CMP-2-keto-3-deoxyoctulosonic acid synthase; Short=CKS; Short=CMP-KDO synthase;
end case
Gene name	Name=kdsB;

Comments [?]

case <OC:Shewanella>
FUNCTION	Activates KDO8N (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in the Shewanella genus.
CATALYTIC ACTIVITY	Reaction=8-amino-3,8-dideoxy-alpha-D-manno-octulosonate + CTP = CMP-8- amino-3,8-dideoxy-alpha-D-manno-oct-2-ulosonate + diphosphate; Xref=Rhea:RHEA:49284, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:87091, ChEBI:CHEBI:91089; EC=2.7.7.90;
else
FUNCTION	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.
CATALYTIC ACTIVITY	Reaction=3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy- beta-D-manno-octulosonate + diphosphate; Xref=Rhea:RHEA:23448, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:85986, ChEBI:CHEBI:85987; EC=2.7.7.38;
PATHWAY	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno- octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3- deoxy-D-manno-octulosonate and CTP: step 1/1.
end case
case defined <Property:Membrane> and <Property:Membrane=2>
PATHWAY	Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
end case
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the KdsB family.

Keywords [?]

Gene Ontology [?]

case <OCellular component:Shewanella>
GO:0016779; Molecular function:nucleotidyltransferase activity
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0008690; Molecular function:3-deoxy-manno-octulosonate cytidylyltransferase activity
GO:0033468; Biological process:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
end case
GO:0009103; Biological process:lipopolysaccharide biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

NCBIfam

TIGR00466; KdsB; 1;

Features [?]

Additional information [?]

Size range	230-310 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	kpsU from E.coli (KPSU5_ECOLX) is not a duplicate, but a kdsB from a non-K12 strain. Kdo8N is found in lipopolysaccharides of members of the Shewanella genus. (PubMed:23413030)