HAMAP annotation rule MF_00059
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Accession MF_00059
Dates 25-OCT-2002 (Created)
16-AUG-2010 (Last updated, Version 38)
Name RNApol_bact_RpoA
Scope Bacteria
Plastid
Triggered by



Identifier
RPOA
case not <OG:Chloroplast>
Protein name
RecName: Full=DNA-directed RNA polymerase subunit alpha;
Short=RNAP subunit alpha;
EC=2.7.7.6;
AltName: Full=RNA polymerase subunit alpha;
AltName: Full=Transcriptase subunit alpha;
end case
case <OG:Chloroplast>
Protein name
RecName: Full=DNA-directed RNA polymerase subunit alpha;
Short=PEP;
EC=2.7.7.6;
AltName: Full=Plastid-encoded RNA polymerase subunit alpha;
Short=RNA polymerase subunit alpha;
end case
Gene name
rpoA


FUNCTION DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates (By similarity).
CATALYTIC ACTIVITY Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
case not <OG:Chloroplast> and not <OC:Cyanobacteria>
SUBUNIT Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).
end case
case <OC:Cyanobacteria>
SUBUNIT In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).
end case
case <OG:Chloroplast>
SUBUNIT In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription (By similarity).
SUBCELLULAR LOCATION Plastid, chloroplast.
end case
DOMAIN The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements (By similarity).
SIMILARITY Belongs to the RNA polymerase alpha chain family.


Transferase
Nucleotidyltransferase
Transcription
DNA-directed RNA polymerase


GO:0003677; Molecular function: DNA binding.
GO:0003899; Molecular function: DNA-directed RNA polymerase activity.
GO:0006351; Biological process: transcription, DNA-dependent.
case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case


Pfam PF01000; RNA_pol_A_bac; 1;
PF03118; RNA_pol_A_CTD; 1;


From: RPOA_ECOLI (P0A7Z4)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     235       Alpha N-terminal domain (alpha-NTD) (By similarity)        
REGION     249     Cter       Alpha C-terminal domain (alpha-CTD) (By similarity)        


Size range: 309-383 amino acids
Related Rules: None
Templates: P0A7Z4 (RPOA_ECOLI); Q925Z2 (RPOA_RHIME); Q9KWU8 (RPOA_THEAQ); Q9Z9H6 (RPOA_THETH): [Recover all]
Fusion: None
Comments: Divergent BIGNA, CARRP, CHLRE, CHLVU, HELSJ, NEPOL, ONYPE, OLTVI, PELHO, PSEAK, SCEOB, STIHE and Euglids not used in alignment; they are either quite short or have inserts. In PELHO there are 3 rpoA-like genes, but it is not clear if any of them are functional.


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