HAMAP rule MF_00059
General rule information
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| Accession | MF_00059 |
| Dates | 25-OCT-2002 (Created)
1-JUN-2023 (Last updated, Version 48) |
| Name | RNApol_bact_RpoA |
| Scope(s) |
Bacteria Plastid |
| Template(s) | P0A7Z4 (RPOA_ECOLI); Q925Z2 (RPOA_RHIME); Q9KWU8 (RPOA_THEAQ); Q9Z9H6 (RPOA_THETH); [ Recover all ] |
| Triggered by |
HAMAP; MF_00059 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RPOA |
| case not <OG:Chloroplast> | |
| Protein name | RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=RNAP subunit alpha; EC=2.7.7.6; AltName: Full=RNA polymerase subunit alpha; AltName: Full=Transcriptase subunit alpha; |
| end case | |
| case <OG:Chloroplast> | |
| Protein name | RecName: Full=DNA-directed RNA polymerase subunit alpha; Short=PEP; EC=2.7.7.6; AltName: Full=Plastid-encoded RNA polymerase subunit alpha; Short=RNA polymerase subunit alpha; |
| end case | |
| Gene name | Name=rpoA; |
Comments
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| FUNCTION | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. |
| CATALYTIC ACTIVITY | Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.6; |
| case not <OG:Chloroplast> and not <OC:Cyanobacteriota> | |
| SUBUNIT | Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
| end case | |
| case <OC:Cyanobacteriota> | |
| SUBUNIT | In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
| end case | |
| case <OG:Chloroplast> | |
| SUBUNIT | In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| DOMAIN | The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements. |
| SIMILARITY | Belongs to the RNA polymerase alpha chain family. |
Keywords
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Gene Ontology
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| GO:0003677; Molecular function:DNA binding | |
| GO:0003899; Molecular function:DNA-directed 5'-3' RNA polymerase activity | |
| GO:0006351; Biological process:DNA-templated transcription | |
| case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
Cross-references
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Features
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| From: RPOA_ECOLI (P0A7Z4) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 235 | /note="Alpha N-terminal domain (alpha-NTD)" | |||||||||
| REGION | 249 | Cter | /note="Alpha C-terminal domain (alpha-CTD)" | |||||||||
Additional information
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| Size range | 309-383 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Divergent BIGNA, CARRP, CHLRE, CHLVU, HELSJ, NEPOL, ONYPE, OLTVI, PELHO, PSEAK, SCEOB, STIHE and Euglids not used in alignment; they are either quite short or have inserts. In PELHO there are 3 rpoA-like genes, but it is not clear if any of them are functional. |