HAMAP rule MF_00061
General rule information
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Accession | MF_00061 |
Dates | 1-JUN-2001 (Created)
12-MAR-2024 (Last updated, Version 37) |
Name | IspE |
Scope(s) |
Bacteria |
Template(s) | P62615 (ISPE_ECOLI); Q8FI04 (ISPE_ECOL6); P83700 (ISPE_THET8); [ Recover all ] |
Triggered by |
HAMAP; MF_00061 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | ISPE |
case <OC:Staphylococcus> or <OC:Streptococcus> | |
Protein name | RecName: Full=Putative 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; Short=CMK; EC=2.7.1.148; AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase; |
else | |
Protein name | RecName: Full=4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; Short=CMK; EC=2.7.1.148; AltName: Full=4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase; |
Gene name | Name=ispE; |
end case |
Comments
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FUNCTION | Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol. |
CATALYTIC ACTIVITY | Reaction=4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D- erythritol 2-phosphate + ADP + H(+); Xref=Rhea:RHEA:18437, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57823, ChEBI:CHEBI:57919, ChEBI:CHEBI:456216; EC=2.7.1.148; |
case not <OC:Staphylococcus> and not <OC:Streptococcus> | |
PATHWAY | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 3/6. |
end case | |
case <OC:Enterobacterales> | |
SUBUNIT | Homodimer. |
end case | |
SIMILARITY | Belongs to the GHMP kinase family. IspE subfamily. |
Keywords
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ATP-binding | |
Nucleotide-binding | |
case not <OC:Staphylococcus> and not <OC:Streptococcus> | |
Isoprene biosynthesis | |
end case | |
Kinase | |
Transferase |
Gene Ontology
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GO:0050515; Molecular function:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity | |
GO:0005524; Molecular function:ATP binding | |
case not <OCellular component:Staphylococcus> and not <OC:Streptococcus> | |
GO:0019288; Biological process:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway | |
end case |
Cross-references
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Pfam | PF08544; GHMP_kinases_C; 1; |
Pfam | PF00288; GHMP_kinases_N; 1; |
PIRSF | PIRSF010376; IspE; 1; |
NCBIfam | TIGR00154; IspE; 1; |
Features
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From: ISPE_ECOL6 (Q8FI04) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 99 | 109 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[PL]-x-x-[GSA]-G-[LIMFV]-[GA]-[GS]-[GAD]-S-[STAG] | ||||||||
ACT_SITE | 10 | 10 | K | |||||||||
ACT_SITE | 141 | 141 | D |
Additional information
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Size range | 255-329 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |
Comments | In CHLPN there is a remnant of an ispE gene but which is interrupted by a probable natural frameshift (all CHLPN strains have it). It is orf CP0905 |