HAMAP

Accession	MF_00071
Dates	1-JUN-2001 (Created) 11-MAY-2011 (Last updated, Version 20)

Data class

Protein

Predictors

HAMAP; MF_00071; [distribution of match scores in UniProtKB];[seed alignment for MF_00071]

Names

LepA

Identifier

LEPA

Protein name

RecName: Full=Elongation factor 4; Short=EF-4; EC=3.6.5.n1; AltName: Full=Ribosomal back-translocase LepA;

Gene name

lepA

FUNCTION: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner (By similarity).

CATALYTIC ACTIVITY: GTP + H(2)O = GDP + phosphate.

SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).

SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).

SIMILARITY: Belongs to the GTP-binding elongation factor family. LepA subfamily.

Pfam	PF00679; EFG_C; 1; PF00009; GTP_EFTU; 1; PF03144; GTP_EFTU_D2; 1; PF06421; LepA_C; 1;
PRINTS	PR00315; ELONGATNFCT; 1;
TIGRFAMs	TIGR01393; LepA; 1; TIGR00231; Small_GTP; 1;
PROSITE	PS00301; EFACTOR_GTP; 1;

Cell membrane.

Cell inner membrane.

GTP-binding, Hydrolase, Membrane, Nucleotide-binding, Protein biosynthesis.

GO:0005886; Cellular component: plasma membrane.
GO:0005525; Molecular function: GTP binding.
GO:0003924; Molecular function: GTPase activity.
GO:0043022; Molecular function: ribosome binding.
GO:0003746; Molecular function: translation elongation factor activity.
GO:0045727; Biological process: positive regulation of translation.

From: LEPA_AQUAE (O67618)
Key		From		To		Description		Condition		FTGroup
NP_BIND		16		21		GTP (By similarity)		[DN]-H-G-K-[ST]-T
NP_BIND		133		136		GTP (By similarity)		[NS]-K-[LIVMCA]-D

Size range:	593-660 amino acids
Related UniRules:	MF_04138 (GUFP (supersedes the current rule))
Template:	P60785 (LEPA_ECOLI); O67618 (LEPA_AQUAE); Q2FXY7 (LEPA_STAA8): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in LACPL, METFK, RHOBA
Plasmid encoded:	None