HAMAP rule MF_00083
General rule information
[?]
| Accession | MF_00083 |
| Dates | 1-JUN-2001 (Created)
23-SEP-2024 (Last updated, Version 26) |
| Name | Pept_tRNA_hydro_bact |
| Scope(s) |
Bacteria |
| Template(s) | P0A7D1 (PTH_ECOLI); P37470 (PTH_BACSU); Q6YP15 (PTH_STAAU); Q9HVC3 (PTH_PSEAE); [ Recover all ] |
| Triggered by |
HAMAP; MF_00083 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | PTH |
| Protein name | RecName: Full=Peptidyl-tRNA hydrolase; Short=Pth; EC=3.1.1.29; |
| Gene name | Name=pth; |
Comments
[?]
| FUNCTION | Hydrolyzes ribosome-free peptidyl-tRNAs (with 1 or more amino acids incorporated), which drop off the ribosome during protein synthesis, or as a result of ribosome stalling. |
| FUNCTION | Catalyzes the release of premature peptidyl moieties from peptidyl-tRNA molecules trapped in stalled 50S ribosomal subunits, and thus maintains levels of free tRNAs and 50S ribosomes. |
| CATALYTIC ACTIVITY | Reaction=an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+); Xref=Rhea:RHEA:54448, Rhea:RHEA-COMP:10123, Rhea:RHEA-COMP:13883, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:59874, ChEBI:CHEBI:78442, ChEBI:CHEBI:138191; EC=3.1.1.29; |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the PTH family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0004045; Molecular function:aminoacyl-tRNA hydrolase activity |
| GO:0005737; Cellular component:cytoplasm |
| GO:0000049; Molecular function:tRNA binding |
| GO:0006515; Biological process:protein quality control for misfolded or incompletely synthesized proteins |
| GO:0072344; Biological process:rescue of stalled ribosome |
Cross-references
[?]
| Pfam | PF01195; Pept_tRNA_hydro; 1; |
| NCBIfam | TIGR00447; Pth; 1; |
| PROSITE | PS01195; PEPT_TRNA_HYDROL_1; 1; |
| PROSITE | PS01196; PEPT_TRNA_HYDROL_2; 1; |
Features
[?]
| From: PTH_ECOLI (P0A7D1) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 21 | 21 | /note="Proton acceptor" | H | ||||||||
| BINDING | 16 | 16 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" |
[YFH] | ||||||||
| BINDING | 67 | 67 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" |
[FYM] | ||||||||
| BINDING | 69 | 69 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" |
N | ||||||||
| BINDING | 115 | 115 | /ligand="tRNA" /ligand_id="ChEBI:CHEBI:17843" |
N | ||||||||
| SITE | 11 | 11 | /note="Discriminates between blocked and unblocked aminoacyl-tRNA" | N | ||||||||
| SITE | 94 | 94 | /note="Stabilizes the basic form of H active site to accept a proton" | D | ||||||||
Additional information
[?]
| Size range | 172-250 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Originally called spoVC in BACSU. Reviewed in 2024, see PubMedID 38927071. |