HAMAP rule MF_00087
General rule information
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| Accession | MF_00087 |
| Dates | 1-JUN-2001 (Created)
15-FEB-2024 (Last updated, Version 51) |
| Name | Glu_tRNA_reductase |
| Scope(s) |
Bacteria Archaea Plastid |
| Template(s) | P0A6X1 (HEM1_ECOLI); Q9UXR8 (HEM1_METKA); P28462 (HEM1_CHLP8); P42809 (HEM1_METTM); P28463 (HEM1_SYNY3); Q59292 (HEMA_RUMJO); P42808 (HEM1_XANCH); [ Recover all ] |
| Triggered by |
HAMAP; MF_00087 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | HEM1 |
| Protein name | RecName: Full=Glutamyl-tRNA reductase; Short=GluTR; EC=1.2.1.70; |
| Gene name | Name=hemA; |
Comments
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| FUNCTION | Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). |
| CATALYTIC ACTIVITY | Reaction=(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L- glutamyl-tRNA(Glu) + NADPH; Xref=Rhea:RHEA:12344, Rhea:RHEA- COMP:9663, Rhea:RHEA-COMP:9680, ChEBI:CHEBI:15378, ChEBI:CHEBI:57501, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78442, ChEBI:CHEBI:78520; EC=1.2.1.70; |
| PATHWAY | Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2. |
| case <Property:PHOTOSYN> | |
| PATHWAY | Porphyrin-containing compound metabolism; chlorophyll biosynthesis. |
| end case | |
| SUBUNIT | Homodimer. |
| case <OG:Chloroplast> | |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| DOMAIN | Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization. |
| MISCELLANEOUS | During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA. |
| SIMILARITY | Belongs to the glutamyl-tRNA reductase family. |
Keywords
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| Porphyrin biosynthesis | |
| case <Property:PHOTOSYN> | |
| Chlorophyll biosynthesis | |
| end case | |
| Oxidoreductase | |
| NADP | |
Gene Ontology
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| GO:0008883; Molecular function:glutamyl-tRNA reductase activity | |
| case <Property:PHOTOSYN> | |
| GO:0015995; Biological process:chlorophyll biosynthetic process | |
| end case | |
| GO:0006782; Biological process:protoporphyrinogen IX biosynthetic process | |
| case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
Cross-references
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Features
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| From: HEM1_METKA (Q9UXR8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 174 | 179 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
|||||||||
| BINDING | 47 | 50 | /ligand="substrate" | T-C-x-R | ||||||||
| BINDING | 99 | 101 | /ligand="substrate" | [ED]-x-[EDQ] | ||||||||
| ACT_SITE | 48 | 48 | /note="Nucleophile" | C | ||||||||
| BINDING | 94 | 94 | /ligand="substrate" | S | ||||||||
| BINDING | 105 | 105 | /ligand="substrate" | Q | ||||||||
| SITE | 84 | 84 | /note="Important for activity" | H | ||||||||
Additional information
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| Size range | 329-497 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | STRAW and STRCO have an internal inserted domains of about 120 residues; sequence not included in alignment. Highly divergent RUMJO; sequence not included in alignment SORC5 has a long C-terminus; not included in alignment |