HAMAP

Accession	MF_00094
Dates	1-JUN-2001 (Created) 28-OCT-2011 (Last updated, Version 18)

Data class

Protein

Predictors

HAMAP; MF_00094; [distribution of match scores in UniProtKB];[seed alignment for MF_00094]

Names

Rel_fac_2

Identifier

RF2

Protein name

RecName: Full=Peptide chain release factor 2; Short=RF-2;

Gene name

prfB

FUNCTION: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

PTM: Methylated by PrmC. Methylation increases the termination efficiency of RF2 (By similarity).

SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor family.

Pfam	PF00472; RF-1; 1;
TIGRFAMs	TIGR00020; PrfB; 1;
PROSITE	PS00745; RF_PROK_I; 1;

Cytoplasm, Protein biosynthesis.

Methylation.

GO:0016149; Molecular function: translation release factor activity, codon specific.
GO:0006415; Biological process: translational termination.
GO:0005737; Cellular component: cytoplasm.

From: RF2_ECOLI (P07012)
Key		From		To		Description		Condition		FTGroup
MOD_RES		252		252		N5-methylglutamine (By similarity)		Q

Size range:	359-377 amino acids
Related UniRules:	MF_00093 (RF1)
Template:	P07012 (RF2_ECOLI)
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	In about 70% of bacterial genomes the mRNA encoding RF-2 has 2 partially overlapping reading frames. Synthesis of full-length RF-2 requires +1 frameshifting at the end of the first open reading frame, usually on a UGA codon. This frameshifting serves an autoregulatory function; when RF-2 levels are low frameshifting occurs and full-length protein is produced.