HAMAP

Accession	MF_00097
Dates	1-JUN-2001 (Created) 13-DEC-2011 (Last updated, Version 38)

Data class

Protein

Predictors

HAMAP; MF_00097; [distribution of match scores in UniProtKB];[seed alignment for MF_00097]

Names

TMP_synthase

Identifier

THIE

Protein name

RecName: Full=Thiamine-phosphate synthase; Short=TP synthase; Short=TPS; EC=2.5.1.3; AltName: Full=Thiamine-phosphate pyrophosphorylase; Short=TMP pyrophosphorylase; Short=TMP-PPase;

Gene name

thiE

FUNCTION: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) (By similarity).

CATALYTIC ACTIVITY: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.

SIMILARITY: Belongs to the thiamine-phosphate synthase family.

Pfam	PF02581; TMP-TENI; 1;
TIGRFAMs	TIGR00693; ThiE; 1;

Thiamine biosynthesis, Transferase.

Magnesium, Metal-binding.

GO:0000287; Molecular function: magnesium ion binding.
GO:0004789; Molecular function: thiamine-phosphate diphosphorylase activity.
GO:0009228; Biological process: thiamine biosynthetic process.

From: THIE_BACSU (P39594)
Key		From		To		Description		Condition		FTGroup
REGION		44		48		HMP-PP binding (By similarity)		Q-x-R-x-[KE]
REGION		143		145		THZ-P binding (By similarity)		[TS]-x-[TS]
REGION (Optional)		195		196		THZ-P binding (By similarity)		[IVL]-[ST]
METAL		80		80		Magnesium (By similarity)		[DE]		1
METAL		99		99		Magnesium (By similarity)		[DE]		1
BINDING		79		79		HMP-PP (By similarity)		[ND]
BINDING		117		117		HMP-PP (By similarity)		[ST]
BINDING		146		146		HMP-PP (By similarity)		[KH]
BINDING		175		175		THZ-P; via amide nitrogen (By similarity)		[GA]

Size range:	204-240 amino acids
Related UniRules:	MF_01327 (THIE (supersedes the current rule))
Template:	P39594 (THIE_BACSU); P30137 (THIE_ECOLI); P66916 (THIE_MYCTU): [Recover all]
Scope:	Bacteria; except Cyanobacteria Archaea
Fusion:	Nter: MF_00228 (thiM); Cter: MF_00089 (thiC), <thiD>, <Unknown>
Duplicate:	in AQUAE, COREF, GEOSL, STRPN, STRR6
Plasmid encoded:	in RHIET
Comments:	Unknown N-terminal domain in Cyanobacteria, this has now been put into a separate family, MF_01327. There is a second copy of ThiE in AQUAE (AQ_1366) and in GEOSL (GSU0587) that lacks the second magnesium binding site. Fusion with ThiC in BIFLO, with ThiD and an unknown domain in COREF, CORGL, with ThiD in one copy in GEOSL, with ThiM in SYMTH. There is a ThiE-like protein in BACSU: TenI, which has a different activity.