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HAMAP annotation rule: MF_00098
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General rule information

Accession MF_00098
Dates 29-OCT-2002 (Created)
21-NOV-2011 (Last updated, Version 33)
Data class Protein
Predictors HAMAP; MF_00098; [distribution of match scores in UniProtKB];[seed alignment for MF_00098]
Names Met_tRNA_synth_type1


Propagated annotation

Identifier, protein and gene names
Identifier SYM
Protein name
RecName: Full=Methionine--tRNA ligase;
EC=6.1.1.10;
AltName: Full=Methionyl-tRNA synthetase;
Short=MetRS;
Gene name metG
Comments
FUNCTION: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity).
CATALYTIC ACTIVITY: ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

case <FTGroup:1>
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
end case


case <Feature:PS50886>
SUBUNIT: Homodimer (By similarity).
end case


case not <Feature:PS50886> and <OC:Bacteria>
SUBUNIT: Monomer (By similarity).
end case

SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

case <Feature:PS50886>
SIMILARITY: Contains 1 tRNA-binding domain.
end case

Cross-references
Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR01041; TRNASYNTHMET; 1;
TIGRFAMs TIGR00398; MetG; 1;
TIGR00399; MetG_C_term; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
PS50886; TRBD; 0-1; trigger=PRU00209;
Keywords
Cytoplasm, Aminoacyl-tRNA synthetase, Protein biosynthesis, Ligase, ATP-binding, Nucleotide-binding.

case <Feature:PS50886>
RNA-binding, tRNA-binding.
end case


case <FTGroup:1>
Metal-binding, Zinc.
end case

Gene Ontology
GO:0005524; Molecular function: ATP binding.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.
Features
From: SYM_ECOLI (P00959)
Key     From     To       Description   Condition   FTGroup
MOTIF     15     25       "HIGH" region   [PYA]-[YT]-x(5)-H-[LIVFA]-G-[HNST]  
MOTIF     333     337       "KMSKS" region   [KTQ]-[LIMF]-[SG]-[KTS]-[ST]  
 
METAL     146     146       Zinc (By similarity)   C   1
METAL     149     149       Zinc (By similarity)   C   1
METAL     159     159       Zinc (By similarity)   C   1
METAL     162     162       Zinc (By similarity)   C   1
 
BINDING     336     336       ATP (By similarity)   [KST]  


Additional information

Size range: 536-734 amino acids
Related UniRules: MF_01228 (SYM)
Template: P00959 (SYM_ECOLI)
Scope: Bacteria
Archaea
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: in ACAM1
Comments: It is possible that several species such as ARCFU, METAC, METMA, METKA, METTH, METJA and TREPA bind a second zinc ion, as their sequences contain a second zinc finger motif corresponding to that of metG subfamily 2

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UniProtKB rule member sequences