HAMAP rule MF_00098
General rule information
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Accession | MF_00098 |
Dates | 29-OCT-2002 (Created)
1-JUN-2023 (Last updated, Version 42) |
Name | Met_tRNA_synth_type1 |
Scope(s) |
Bacteria Archaea |
Template(s) | P00959 (SYM_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_00098 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | SYM |
Protein name | RecName: Full=Methionine--tRNA ligase; EC=6.1.1.10; AltName: Full=Methionyl-tRNA synthetase; Short=MetRS; |
Gene name | Name=metG; |
Comments
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FUNCTION | Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation. |
CATALYTIC ACTIVITY | Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L- methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667, Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530, ChEBI:CHEBI:456215; EC=6.1.1.10; |
case <FTGroup:1> | |
COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
end case | |
case <Feature:PS50886> | |
SUBUNIT | Homodimer. |
end case | |
case not <Feature:PS50886> and <OC:Bacteria> | |
SUBUNIT | Monomer. |
end case | |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. |
Keywords
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Cytoplasm | |
Aminoacyl-tRNA synthetase | |
Protein biosynthesis | |
Ligase | |
ATP-binding | |
Nucleotide-binding | |
case <Feature:PS50886> | |
RNA-binding | |
tRNA-binding | |
end case | |
case <FTGroup:1> | |
Metal-binding | |
Zinc | |
end case |
Gene Ontology
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GO:0005524; Molecular function:ATP binding |
GO:0004825; Molecular function:methionine-tRNA ligase activity |
GO:0006431; Biological process:methionyl-tRNA aminoacylation |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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Pfam | PF00133; tRNA-synt_1; 1; |
PRINTS | PR01041; TRNASYNTHMET; 1; |
NCBIfam | TIGR00398; MetG; 1; |
NCBIfam | TIGR00399; MetG_C_term; 1; |
PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
PROSITE | PS50886; TRBD; 0-1; |
Features
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From: SYM_ECOLI (P00959) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
MOTIF | 15 | 25 | /note="'HIGH' region" | [PYA]-[YT]-x(5)-H-[LIVFA]-G-[HNST] | ||||||||
MOTIF | 333 | 337 | /note="'KMSKS' region" | [KTQ]-[LIMF]-[SG]-[KTS]-[ST] | ||||||||
BINDING | 146 | 146 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
BINDING | 149 | 149 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
BINDING | 159 | 159 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
BINDING | 162 | 162 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
BINDING | 336 | 336 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
[KST] |
Additional information
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Size range | 536-734 amino acids |
Related rules |
MF_01228 |
Fusion | Nter: None Cter: None |
Comments | It is possible that several species such as ARCFU, METAC, METMA, METKA, METTH, METJA and TREPA bind a second zinc ion, as their sequences contain a second zinc finger motif corresponding to that of metG subfamily 2 |