HAMAP rule MF_00100
General rule information
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| Accession | MF_00100 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 53) |
| Name | IF_2 |
| Scope(s) |
Bacteria Archaea Plastid |
| Template(s) | P04766 (IF2_GEOSE); P0A705 (IF2_ECOLI); P48515 (IF2_THET8); Q9HNQ2 (IF2P_HALSA); O26359 (IF2P_METTH); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria> or <OG:Chloroplast>
HAMAP; MF_00100_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_00100_A (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Bacteria> | |
| Identifier | IF2 |
| Protein name | RecName: Full=Translation initiation factor IF-2; |
| end case | |
| case <OG:Chloroplast> | |
| Identifier | IF2C |
| Protein name | RecName: Full=Translation initiation factor IF-2, chloroplastic; |
| end case | |
| case <OC:Archaea> | |
| Identifier | IF2P |
| Protein name | RecName: Full=Probable translation initiation factor IF-2; |
| end case | |
| Gene name | Name=infB; |
Comments
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| case <OC:Bacteria> | |
| FUNCTION | One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| end case | |
| case <OC:Archaea> | |
| FUNCTION | Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. |
| end case | |
| case <OG:Chloroplast> | |
| FUNCTION | One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. |
| SUBCELLULAR LOCATION | Plastid, chloroplast. |
| end case | |
| SIMILARITY | Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily. |
Keywords
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| case <OC:Bacteria> | |
| Cytoplasm | |
| end case | |
| Initiation factor | |
| Protein biosynthesis | |
| GTP-binding | |
| Nucleotide-binding | |
Gene Ontology
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| GO:0003924; Molecular function:GTPase activity | |
| GO:0003743; Molecular function:translation initiation factor activity | |
| GO:0006413; Biological process:translational initiation | |
| case <OCellular component:Bacteria> | |
| GO:0005737; Cellular component:cytoplasm | |
| end case | |
| case <OG:Chloroplast> | |
| GO:0009507; Cellular component:chloroplast | |
| end case | |
Cross-references
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| Pfam | PF00009; GTP_EFTU; 1; |
| NCBIfam | TIGR00487; IF-2; 1; |
| NCBIfam | TIGR00491; AIF-2; 1; |
| PROSITE | PS51722; G_TR_2; 1; |
| PROSITE | PS01176; IF2; 1; |
Features
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| From: IF2_ECOLI (P0A705) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Bacteria> or <OG:Chloroplast> | ||||||||||||
| BINDING | 398 | 405 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-H-V-D-H-G-K-T | ||||||||
| BINDING | 444 | 448 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-T-P-G-H | ||||||||
| BINDING | 498 | 501 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[NST]-K-[LIVCMT]-D | ||||||||
| REGION | 392 | 540 | /note="G-domain" | |||||||||
| end case | ||||||||||||
| From: IF2P_METTH (O26359) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Archaea> | ||||||||||||
| BINDING | 12 | 19 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
G-H-V-D-[HVA]-G-K-T | ||||||||
| BINDING | 76 | 80 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
D-T-P-G-H | ||||||||
| BINDING | 130 | 133 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[NT]-K-[LIV]-D | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 589-620 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Intein in some members. |