HAMAP

Accession	MF_00103
Dates	1-JUN-2001 (Created) 22-SEP-2011 (Last updated, Version 34)

Data class

Protein

Predictors

HAMAP; MF_00103; [distribution of match scores in UniProtKB];[seed alignment for MF_00103]

Names

Fapy-DNA_glycosyl

Identifier

FPG

Protein name

RecName: Full=Formamidopyrimidine-DNA glycosylase; Short=Fapy-DNA glycosylase; EC=3.2.2.23; AltName: Full=DNA-(apurinic or apyrimidinic site) lyase MutM; Short=AP lyase MutM; EC=4.2.99.18;

Gene name

mutM, fpg

FUNCTION: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).

CATALYTIC ACTIVITY: Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.

CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

COFACTOR: Binds 1 zinc ion per subunit (By similarity).

SUBUNIT: Monomer (By similarity).

SIMILARITY: Belongs to the FPG family.

SIMILARITY: Contains 1 FPG-type zinc finger.

Pfam	PF01149; Fapy_DNA_glyco; 1; PF06827; zf-FPG_IleRS; 1;
TIGRFAMs	TIGR00577; Fpg; 1;
PROSITE	PS51068; FPG_CAT; 1; PS01242; ZF_FPG_1; 1; PS51066; ZF_FPG_2; 1; trigger=PRU00391;

DNA damage, DNA repair, DNA-binding, Hydrolase, Glycosidase, Lyase, Multifunctional enzyme.

Metal-binding, Zinc, Zinc-finger.

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) lyase activity.
GO:0008534; Molecular function: oxidized purine base lesion DNA N-glycosylase activity.

GO:0008270; Molecular function: zinc ion binding.

GO:0006281; Biological process: DNA repair.

From: FPG_ECOLI (P05523)
Key		From		To		Description		Condition		FTGroup
INIT_MET		1		1		Removed (By similarity)
ACT_SITE		2		2		Schiff-base intermediate with DNA (By similarity)		P
ACT_SITE		3		3		Proton donor (By similarity)		E
ACT_SITE		57		57		Proton donor; for beta-elimination activity (By similarity)		K
ACT_SITE		259		259		Proton donor; for delta-elimination activity (By similarity)		R
BINDING		90		90		DNA (By similarity)		H
BINDING		109		109		DNA (By similarity)		R
BINDING		150		150		DNA (By similarity)		[KR]

Size range:	262-309 amino acids
Related UniRules:	None
Template:	P05523 (FPG_ECOLI); P42371 (FPG_LACLC); O50606 (FPG_THET8): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	Weird insert in DESVH (not taken into account in size range)