HAMAP annotation rule MF_00103
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Accession MF_00103
Dates 1-JUN-2001 (Created)
25-JUL-2012 (Last updated, Version 36)
Name Fapy-DNA_glycosyl
Scope Bacteria
Triggered by



Identifier
FPG
Protein name
RecName: Full=Formamidopyrimidine-DNA glycosylase;
Short=Fapy-DNA glycosylase;
EC=3.2.2.23;
AltName: Full=DNA-(apurinic or apyrimidinic site) lyase MutM;
Short=AP lyase MutM;
EC=4.2.99.18;
Gene name
mutM, fpg


FUNCTION Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).
CATALYTIC ACTIVITY Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
COFACTOR Binds 1 zinc ion per subunit (By similarity).
SUBUNIT Monomer (By similarity).
SIMILARITY Belongs to the FPG family.
case <Feature:PS51066>
SIMILARITY Contains 1 FPG-type zinc finger.
end case


DNA damage
DNA repair
DNA-binding
Hydrolase
Glycosidase
Lyase
Multifunctional enzyme
case <Feature:PS51066>
Metal-binding
Zinc
Zinc-finger
end case


GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) lyase activity.
GO:0008534; Molecular function: oxidized purine nucleobase lesion DNA N-glycosylase activity.
case <Feature:PS51066>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006281; Biological process: DNA repair.


Pfam PF01149; Fapy_DNA_glyco; 1;
PF06827; zf-FPG_IleRS; 1;
TIGRFAMs TIGR00577; Fpg; 1;
PROSITE PS51068; FPG_CAT; 1;
PS01242; ZF_FPG_1; 1;
PS51066; ZF_FPG_2; 1; trigger=PRU00391;


From: FPG_ECOLI (P05523)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     2     2       Schiff-base intermediate with DNA (By similarity)     P  
ACT_SITE     3     3       Proton donor (By similarity)     E  
ACT_SITE     57     57       Proton donor; for beta-elimination activity (By similarity)     K  
ACT_SITE     259     259       Proton donor; for delta-elimination activity (By similarity)     R  
BINDING     90     90       DNA (By similarity)     H  
BINDING     109     109       DNA (By similarity)     R  
BINDING     150     150       DNA (By similarity)     [KR]  


Size range: 262-309 amino acids
Related Rules: None
Templates: P05523 (FPG_ECOLI); P42371 (FPG_LACLC); O50606 (FPG_THET8): [Recover all]
Fusion: None
Comments: Weird insert in DESVH (not taken into account in size range)


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