 |
|
| HAMAP annotation rule: MF_00103 |
| Accession |
MF_00103 |
| Dates |
1-JUN-2001 (Created) 22-SEP-2011 (Last updated, Version 34) |
| Protein name |
| RecName: |
Full=Formamidopyrimidine-DNA glycosylase; Short=Fapy-DNA glycosylase; EC=3.2.2.23; |
| AltName: |
Full=DNA-(apurinic or apyrimidinic site) lyase MutM; Short=AP lyase MutM; EC=4.2.99.18; |
|
FUNCTION: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).
CATALYTIC ACTIVITY: Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
SUBUNIT: Monomer (By similarity).
SIMILARITY: Belongs to the FPG family.
case <Feature:PS51066>
SIMILARITY: Contains 1 FPG-type zinc finger.
end case
case <Feature:PS51066>
end case
GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) lyase activity.
GO:0008534; Molecular function: oxidized purine base lesion DNA N-glycosylase activity.
case <Feature:PS51066>
end case
| From: FPG_ECOLI (P05523) |
| Key |
|
From |
|
To |
|
Description |
|
Condition |
|
FTGroup |
| INIT_MET |
|
1 |
|
1 |
|
Removed (By similarity) |
|
|
|
|
| ACT_SITE |
|
2 |
|
2 |
|
Schiff-base intermediate with DNA (By similarity) |
|
P |
|
|
| ACT_SITE |
|
3 |
|
3 |
|
Proton donor (By similarity) |
|
E |
|
|
| ACT_SITE |
|
57 |
|
57 |
|
Proton donor; for beta-elimination activity (By similarity) |
|
K |
|
|
| ACT_SITE |
|
259 |
|
259 |
|
Proton donor; for delta-elimination activity (By similarity) |
|
R |
|
|
| BINDING |
|
90 |
|
90 |
|
DNA (By similarity) |
|
H |
|
|
| BINDING |
|
109 |
|
109 |
|
DNA (By similarity) |
|
R |
|
|
| BINDING |
|
150 |
|
150 |
|
DNA (By similarity) |
|
[KR] |
|
|
| Size range: |
262-309 amino acids |
| Related UniRules: |
None |
| Template: |
P05523 (FPG_ECOLI); P42371 (FPG_LACLC); O50606 (FPG_THET8): [Recover all] |
| Scope: |
Bacteria |
| Fusion: |
Nter: None; Cter: None |
| Duplicate: |
None |
| Plasmid encoded: |
None |
| Comments: |
Weird insert in DESVH (not taken into account in size range) |
View rule in raw text format (no links)