HAMAP

Accession	MF_00107
Dates	1-JUN-2001 (Created) 16-MAY-2011 (Last updated, Version 29)

Data class

Protein

Predictors

HAMAP; MF_00107; [distribution of match scores in UniProtKB];[seed alignment for MF_00107]

Names

IspF

Identifier

ISPF

Protein name

RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; Short=MECDP-synthase; Short=MECPS; EC=4.6.1.12;

Gene name

ispF

FUNCTION: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (By similarity).

CATALYTIC ACTIVITY: 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP.

COFACTOR: Binds 1 divalent metal cation per subunit (By similarity).

PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

SUBUNIT: Homotrimer (By similarity).

SIMILARITY: Belongs to the IspF family.

Pfam	PF02542; YgbB; 1;
TIGRFAMs	TIGR00151; IspF; 1;
PROSITE	PS01350; ISPF; 1;

Isoprene biosynthesis, Lyase, Metal-binding.

GO:0008685; Molecular function: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.

From: ISPF_ECOLI (P62617)
Key		From		To		Description		Condition		FTGroup
METAL		8		8		Divalent metal cation (By similarity)		D
METAL		10		10		Divalent metal cation (By similarity)		H
METAL		42		42		Divalent metal cation (By similarity)		H
SITE		34		34		Transition state stabilizer (By similarity)		[HDN]
SITE		133		133		Transition state stabilizer (By similarity)		[TS]

Size range:	152-184 amino acids
Related UniRules:	MF_01520 (ISPDF (supersedes the current rule))
Template:	P62617 (ISPF_ECOLI); P44815 (ISPF_HAEIN); Q8RQP5 (ISPF_THET8): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	See MF_01520 for bifunctional ispDF