HAMAP

Accession	MF_00109
Dates	1-JUN-2001 (Created) 28-JAN-2010 (Last updated, Version 34)

Data class

Protein

Predictors

HAMAP; MF_00109; [distribution of match scores in UniProtKB];[seed alignment for MF_00109]

Names

Shikimate_kinase

Identifier

AROK

Protein name

RecName: Full=Shikimate kinase; Short=SK; EC=2.7.1.71;

Protein name

RecName: Full=Shikimate kinase 1; Short=SK 1; EC=2.7.1.71;

Gene name

aroK

FUNCTION: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate (By similarity).

CATALYTIC ACTIVITY: ATP + shikimate = ADP + shikimate 3-phosphate.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the shikimate kinase family.

Pfam	PF01202; SKI; 1;
PRINTS	PR01100; SHIKIMTKNASE; 1;
PROSITE	PS01128; SHIKIMATE_KINASE; 1;

Cytoplasm, Amino-acid biosynthesis, Aromatic amino acid biosynthesis, ATP-binding, Kinase, Magnesium, Metal-binding, Nucleotide-binding, Transferase.

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004765; Molecular function: shikimate kinase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: AROK_MYCTU (P0A4Z2)
Key		From		To		Description		Condition		FTGroup
NP_BIND		12		17		ATP (By similarity)
METAL		16		16		Magnesium (By similarity)		[ST]
BINDING		34		34		Substrate (By similarity)		D
BINDING		58		58		Substrate (By similarity)		R
BINDING		80		80		Substrate; via amide nitrogen (By similarity)		G
BINDING		117		117		ATP (By similarity)		[RK]
BINDING		136		136		Substrate (By similarity)		R
BINDING (Optional)		153		153		ATP (By similarity)		[RQ]

Size range:	161-214 amino acids
Related UniRules:	MF_01269 (AROL (supersedes the current rule))
Template:	P0A6D7 (AROK_ECOLI); P0A4Z2 (AROK_MYCTU): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: MF_00110 (aroB)
Duplicate:	None
Plasmid encoded:	None
Comments:	Archaeal aroK belong to a separate family (MF_00370). Enterobacteriaceae (except WIGBR, BLOBP, BLOFL, BUCAI, BUCAP and BUCBP) possess a second shikimate kinase (aroL, MF_01269). Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain. Strange longer C-terminus in FRASC; sequence not included in size range.