HAMAP

Accession	MF_00121
Dates	1-JUN-2001 (Created) 11-AUG-2011 (Last updated, Version 23)

Data class

Protein

Predictors

HAMAP; MF_00121; [distribution of match scores in UniProtKB];[seed alignment for MF_00121]

Names

GatB

Identifier

GATB

Protein name

RecName: Full=Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Short=Asp/Glu-ADT subunit B; EC=6.3.5.-;

Gene name

gatB

FUNCTION: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).

CATALYTIC ACTIVITY: ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.

CATALYTIC ACTIVITY: ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.

SUBUNIT: Heterotrimer of A, B and C subunits (By similarity).

SIMILARITY: Belongs to the GatB/GatE family. GatB subfamily.

Pfam	PF02934; GatB_N; 1; PF02637; GatB_Yqey; 1;
TIGRFAMs	TIGR00133; GatB; 1;
PROSITE	PS01234; GATB; 1;

ATP-binding, Ligase, Nucleotide-binding, Protein biosynthesis.

GO:0050567; Molecular function: glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity.
GO:0006412; Biological process: translation.

Size range:	449-517 amino acids
Related UniRules:	MF_00588 (GATE)
Template:	O30509 (GATB_BACSU); Q9LCX2 (GATB_THET8); O27341 (GATB_METTH): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: MF_00126 (glnS); Cter: None
Duplicate:	in SYNAS
Plasmid encoded:	None